Abstract Cucumber ( Cucumis sativus L.) is a tropical vegetable and a source of vitamins such as K, C, and B. It is commonly grown and sold for daily consumption, but picking the right fruit size is more profitable. Therefore, a method for estimating the fruit weight is highly recommended. This paper aimed to determine the dimensions of cucumber fruit based on its usual harvesting size and to establish a model to show the relationship between fruit weight, fruit length, and fruit diameter. Cucumber was planted in the experimental field belonging to the Faculty of Agricultural Biosystems Engineering, Royal University of Agriculture, Phnom Penh, Cambodia, from January to June 2022. In the study, 48 market-size fruits were randomly selected from the plots to measure their weight, length, and diameter. The result shows that fruit length and fruit diameter had a positive relationship ( P < 0.001; R = 0.70). Fruit weight was 3.38 fruit length × fruit diameter ( P <0.001; R = 0.95). Nevertheless, L/D ratio negatively affected fruit weight, when it exceeded 3:1. Fruit weight was greater than 100 g when fruit diameter was over 4 cm and fruit length was over 10 cm. Therefore, when picking cucumber fruits, one must consider fruit length and diameter to be profitable. Further studies will focus on measuring cucumber fruit already available on the market to understand more about actual consumer preferences.

Abstract Hydroponics is a modern agricultural system that enables year-round plant growth. Biochar, derived from apple tree waste, and humic acid were investigated as a replacement for the Hoagland nutrient solution to grow strawberries in a greenhouse with three replications. Growth parameters, such as leaf area, the average number of fruits per plant, maximum fruit weight, and the weight of fresh and dry fruits, were measured. A 50% increase in fresh and dry fruit weight was observed in plants grown using biochar compared to the control. Additionally, the use of Hoagland chemical fertilizer led to a 25% increase in both fresh and dry weight. There was a 65% increase in the number of fruits per plant in the biochar-grown sample compared to the control. Moreover, biochar fertilizer caused a 100% increase in maximum fruit weight compared to the control and a 27% increase compared to the Hoagland chemical fertilizer. Biochar had a higher pH compared to the Hoagland solution, and such pH levels were conducive to strawberry plant growth. The results indicate that biochar has the potential to enhance the size and weight of fruits. The findings of the study demonstrate that biochar, when combined with humic acid, is a successful organic hydroponic fertilizer that improves the quality and quantity of strawberries. Moreover, this approach enables the more efficient utilization of garden waste.

Abstract Tomato is one of the major solanaceous vegetables, which has a unique place in the global vegetable market. Instead of being a high-value crop, there is still a need to do improvement in its potential against various biotic and abiotic stressors that adequately demolish its real yield. Alternaria solani (causing early blight disease) is designated as one of the fatal organisms that may reduce tomato crop yield by up to 80%. There were lots of methods, viz., chemical, cultural and biological suggested to overcome it. However, chemical strategies are much in vogue, but they have several negative consequences for human health and the ecosystem. Enlightening this issue, the efficacy of various treatments, viz., chemical fungicides (Amistar Top ® , Nativo ® , and Contaf ® ), biochar and fungal bioagent ( Trichoderma viride ) was assessed under both in vivo and in vitro conditions. Induced resistance is mediated by several regulating pathways, like salicylic acid and jasmonic acid. These mediating pathways manipulate different physiological processes like growth and development, stress tolerance, and defence mechanisms of the plant. The assessment of results revealed that among all treatments biochar at 3.25% by weight consistently displayed remarkable effectiveness against the early blight infection by triggering resistance and improving the overall performance of tomato plants. This result is attributed to improved soil health, fastening mineralization as well as absorption processes, and boosting the plant’s immunity with the use of a higher concentration of biochar. Hence, it could be recommended for the overall improvement of tomato crop and its sustainability.

Abstract Plum ( Prunus domestica ) is a seasonal nutraceutical fruit rich in many functional food nutrients such as vitamin C, antioxidants, total phenolic content, and minerals. Recently, researchers have focused on improvised technologies for the retention of bioactive compounds during the processing of perishable fruits; plum is one of these fruits. This study looked at how the percentage of moisture content and percentage of acidity were affected by conventional drying and osmotic dehydration. Total phenolic content (mg GA/100 g of plum), total anthocyanin content (mg/100 g), and vitamin C (mg/100 g) Conventional drying of fruit was carried out at 80.0 ℃ for 5 h. At various temperatures (45.0 ℃, 50.0 ℃, and 55.0 ℃) and hypertonic solution concentrations (65.0 B, 70.0 B, and 75.0 B), the whole fruit was osmotically dehydrated. It was observed that the osmotically treated fruit retains more nutrients than conventionally dried fruit. The total phenolic content of fruit significantly increased with the increase in process temperature. However, vitamin C and total anthocyanin content of the fruit decreased significantly with process temperature, and hypertonic solution concentration was observed. Hence, it was concluded that osmodehydration could be employed for nutrient retention in plum fruit over conventional drying. This process needs to be further refined, improvised, and optimised for plum processing.

Abstract The present study demonstrates the effect of direct solar drying (DSD) and hot air drying (HAD) on the quality attributes of Fuji apple slices. DSD samples took a longer time (150–180 min) to dry and simultaneously reached higher equilibrium moisture content at the end of rehydration than HAD samples. DSD samples have higher rehydration ability, dry matter holding capacity, and water absorption capacity than HAD samples. Among several empirical models, the Weibull model is the best fit with higher R 2 (0.9977), lower root mean square (0.0029), and chi-square error (0.0031) for describing the rehydration kinetics. Rehydrated HAD samples showed better color characteristics than DSD in terms of overall color change, chroma, and hue angle values. Whereas the hardness and chewiness of rehydrated DSD samples were better than HAD samples because of higher dry matter holding capacity in DSD. Apart from color retention, the DSD samples showed better rehydration capacity and a good texture upon rehydration than HAD slices.

Abstract The H3N2 influenza virus is spiking dramatically, which is a major concern worldwide and in India. The multifunctional hetero-trimer influenza virus RNA-dependent RNA polymerase (RdRP) is involved in the generation of viral mRNA and is crucial for viral infectivity, which is directly related to the virus’s ability to survive. The goal of the current work was to use molecular docking to determine how the RdRP protein might be affected by powerful bioactive chemicals found in Calotropis gigantia latex. By applying CB-dock 2 analysis and 2D interactions, an in-silico docking study was conducted using a GC-FID (gas chromatography with flame-ionization detection) based composition profile. Tocospiro A (15%), Amyrin (7%), and Gombasterol A were found by GC-FID to be the main phytocompounds in the latex of Calotropis gigantia . The docking result showed that ligands were effectively bound to RdRP. According to interaction studies, RdRP/ligand complexes create hydrogen bonds, van der Waals forces, pi-alkyl bonds, alkyl bonds, and pi-Sigma bonds. Therefore, it was suggested that Calotropis gigantia latex may represent a possible herbal remedy to attenuate H3N2 infections based on the above findings of the fragrance profile and docking.

Abstract The most crucial factor in producing papaya seedlings successfully is seed germination. The purpose of this study was to investigate the influence of seed priming with growing media on seed germination and seedling growth of papaya from October to December 2022. The experimental treatments included three seed priming treatments: T 0 = control (no seed priming treatments), T 1 = GA 3 (100 ppm), and T 2 = KNO 3 (1%), and four growing media, viz., M 1 = soil + vermicompost (1:1), M 2 = soil + cowdung (1:1), M 3 = soil + cocopeat + vermicompost (1:1:1), and M 4 = soil + cocopeat + cowdung (1:1:1). The treatments showed a significant effect on different parameters such as germination percentage, days to germination, survival percentage, chlorophyll content, seed vigor index, shoot, and root length. GA 3 treated seedlings performed better than non-GA 3 -treated seedlings. Among the growing media, M 3 showed the best for seed germination and other growth attributes compared to other growing media. In terms of interaction effects, T 1 M 3 showed the highest performance for germination percentage (84.33%), survival percentage (91.0%), and chlorophyll content (44.26%). T 1 M 3 also showed the highest seed vigor index, shoot and root growth, and plant biomass. As a result, the combination of GA 3 and growing media containing soil + cocopeat + vermicompost was shown to be the most favorable for papaya seed germination and seedling growth.

Abstract The aim of the present study was to determine the effects of single and mixed infections of nematode ( Meloidogyne javanica ), fungus ( Fusarium oxysporum ) and bacterium ( Xanthomonas axonopodis ) on nodulation and pathological parameters of Bambara groundnut ( Vigna subterrenea (L.) Verdc.) in field condition. Nematode infested field was used while other pathogens were obtained from diseased plants. The Randomized Complete Block Design (RCBD) was adopted in a 5 × 9 × 5 factorial design (5 blocks, 9 treatments and 5 replicates per treatments) resulting in 225 experimental units. In each experimental unit, three seeds were sown to a depth of 5cm and thinned to one plant per planting hole after germination at day 7. Treatments were inoculated into test plant following standard methods. As a result, the control treatment recorded the highest number of nodules (64.0 ± 6.91), followed by bacterium (45.2 ± 5.11) while N + F + B had the lowest number of root nodules (23.4 ± 2.42). Simultaneous treatment (N + F + B) gave the highest percentage reduction in nodulation (63.44%), followed by treatment N + F7 (56.25%). Fungus treatment recorded the highest mean wilted plants (3.8 + 0.20) followed by N + F7 treatment (3.40 + 0.40). Gall formation in the nematode treatment increased proportionately by 56.33% as the highest recorded, followed by treatment N + F7 with 50.0%. Treatment N + F7 had the highest reproduction factor (Rf) value of 9.30 followed by nematode (8.30), N + B7 (7.40), N + F + B (6.80) and N + F14 (6.50). Zero (0) Rf value was recorded in fungus, bacterium and control treatments. The observed differences in nodulation and pathological parameters among the treatments are significant ( P < 0.05). The data provided in this work is important in the control of the three pathogens affecting the productivity of Bambara nut. Formulation of a single protectant should be designed to have potent effects on the three pathogens to achieve effective protection and good production of Bambara nut.

Abstract We carried out research work to infect the roots of mature pine ( Pinus densiflora ) with Tricholoma matsutake for cultivation of the pine mushroom. Four-month-old and one-year-old seedlings and forty-year-old mature pine were treated with α-NAA (α-naphthalene acetic acid) as rooting agent for generating new rootlets. The optimum concentrations of α-NAA for generating new rootlets in four-month-old and one-year-old pine seedlings were 0.5 mg per root, at which numbers of generated new rootlets were approximately 2–3 times higher than in control. The mature pine treated with 1.0 mg of α-NAA per root produced approximately 1.7 times more new rootlets than untreated. Roots in 15 mature pines were treated with α-NAA, and about 79% of the treated roots successfully generated new branching roots. For mycorrhizal synthesis, the new rootlets without contamination were inoculated with mycelia of matsutake cultured in a glass container with sterilized vermiculite substrate. After 4 months, it was identified by ITS specific primer method that about 50% of the analyzed root samples were infected with matsutake. The results showed that the roots of mature pine can be infected by matsutake.

Abstract Salinity is a significant factor restricting plant development at various stages, resulting in lower yield and productivity. The current study was carried out to investigate and assess the tolerance of several tomato genotypes to salty conditions. Thirty (30) tomato genotypes were cultivated in pots and tested for salinity at three levels: 5 ds/m NaCl, 10 ds/m NaCl, and 15 ds/m NaCl, in comparison to the control (0 mM NaCl). Two weeks after treatment, several morphological and physiological parameters were measured. The effects of salt stress on tomato genotypes included a considerable reduction in leaf area, chlorophyll content, shoot and root length, shoot and root biomass, and relative water content. Different tomato genotypes responded differently to salinity severity score (SSS). Reduction of shoot dry weight (0.27 to 0.44) and leaf area (0.33 to 0.45) were positively correlated with SSS at moderate (10 ds/m) to higher (15 ds/m) salinity levels, respectively. Based on the experiment results, the genotypes BARI Tomato 4, BARI Tomato 14, BARI Tomato 15, SAU Tomato 2, AV 0 T 0 1228, and NS 501 were found to be more salinity tolerant than other genotypes. The results showed that measuring shoot length, leaf area, and shoot fresh and dry weight was better for evaluating salinity stress and screening salt-tolerant tomato genotypes.

Abstract Kinnow production is hampered due to the lack of micronutrient applications such as zinc (Zn), iron (Fe), and manganese (Mn), which play a significant role in the metabolic activities of the plant, affecting yield and quality. The farmers of the region use mineral micronutrient fertilizers, but it leads to phytotoxicity due to unoptimized fertilizer application dose. In the present investigation, an attempt has been made to optimize the Zn, Mn, and Fe minerals dose as tank mix foliar application for improvement of fruit yield, quality, and uptake of nutrients. The twelve combinations of different doses of zinc sulphate, manganese sulphate, and ferrous sulphate fertilizers replicated three times were tested at kinnow orchards established at Krishi Vigyan Kendra, Bathinda, Punjab, India. The data revealed that the fruit drop was significantly low in the treatment F 12 (43.4%) (tank mix spray of 0.3% ZnSO 4 + 0.2% MnSO 4 + 0.1% FeSO 4 ) compared to control treatment. The fruit yield per tree was significantly higher in the treatment F12 compared to untreated control. The juice percentage was also recorded higher in treatment F12 as compared to control, and the juice percentage improved by 2.6%. The leaf nutrient analysis also revealed translocation of higher amount of nutrient from leaf to fruit under optimized supply of micronutrient. Thus, the application of tank mix spray of 0.3% ZnSO 4 + 0.2% MnSO 4 + 0.1% FeSO 4 may be used for better fruit yield and quality.

Abstract The coconut industry has deep historical and economic importance in Sri Lanka, but coconut palms are vulnerable to water stress exacerbated by environmental challenges. This study explored using Sunn hemp ( Crotalaria juncea L.) in major coconut-growing soils in Sri Lanka to improve resilience to water stress. The study was conducted at the Coconut Research Institute of Sri Lanka to evaluate the growth of Sunn hemp in prominent coconut soils—gravel, loamy, and sandy—to determine its cover crop potential. Sunn hemp was planted in pots with the three soil types, arranged in a randomized, complete design with 48 replicates. Growth parameters like plant height, shoot/root dry weight, root length, and leaf area were measured at 2, 4, 6, and 8 weeks after planting. Soil type significantly impacted all growth parameters. After 8 weeks, sandy soil showed the highest plant height and root length, while loamy soil showed the highest shoot/root dry weight and leaf area, followed by sandy and gravel soils. Nitrogen content at 6 and 8 weeks was highest in loamy soil plants. In summary, Sunn hemp produces more biomass in sandy soils, while loamy soils promote greater nutrient accumulation and growth. This suggests the suitability of Sunn hemp as a cover crop across major coconut-growing soils in Sri Lanka, improving resilience.

Abstract Highly nutritive and antioxidants-enriched okra ( Abelmoschus esculentus ) gets sub-optimal field yield due to the irregular germination coupled with non-synchronized harvests. Hence, the research aimed at assessing the combined impact of seed priming and field-level gibberellic acid (GA 3 ) foliar spray on the yield and post-harvest quality of okra. The lab studies were conducted using a complete randomized design (CRD), while the field trials were performed following a factorial randomized complete block design (RCBD) with three replications. Okra seeds were subjected to ten different priming methods to assess their impact on seed germination and seeding vigor. In the premier step, okra seeds were subjected to ten different priming methods, like hydro priming for 6, 12, and 18 h, halo priming with 3% NaCl at 35 ℃, 45 ℃, and 60 ℃, acid priming with 80% H 2 SO 4 for 2.5, 5, and 10 min. Based on the observation, hydro priming for 12 h exhibited the best germination rate (90%), followed by halo seed priming at 60 ℃ and acid priming for 5 min. Furthermore, the halo priming at 60 ℃ demonstrated the greatest seedling vigor index (1965), whereas acid priming for 5 min resulted in favorable outcomes in terms of early emergence in 2.66 days. In addition, varying concentrations of GA 3 (0, 100, 200, and 300 ppm) were also administered to the best three primed seedlings for evaluating their field performance. The findings indicated that applying GA 3 at a concentration of 300 ppm to seedlings raised through acid priming (80% H 2 SO 4 for 5 min) resulted in improved leaf length, reduced time to flowering (first and 50%) and harvest, increased pod diameter, individual pod weight, and yield per plant (735.16 g). Additionally, the treatment involving GA 3 at 300 ppm with halo priming (3% NaCl) at 60 ℃ exhibited the longest shelf life (21 days) of okra with the lowest levels of rotting (6.73%) and color change (1.12) in the polyethylene storage condition.

Abstract Mangifera indica L. (Mango, Anacardiaceae) is a popular tropical evergreen tree known for its nutritional and medicinal values. It is native to India and Southeast Asia and is known as the “king of fruits” in India and the Philippines. It is considered important in Ayurveda and other systems of medicine. Mango fruit is unique in its taste, colour, aroma, and nutritional qualities. Mangoes are a rich source of polyphenols (Mangiferin, Gallotannins, Quercetin, Isoquercetin, Ellagic acid, Glucogallin, Kaempferol, Catechins, Tannins, and the unique Xanthonoid), phenolic acids (Hydroxybenzoic acids- Gallic, Vanillic, Syringic, Protocatechuic, and p -Hydroxybenzoic acids, Hydroxycinnamic acid derivatives- p -Coumaric, Chlorogenic, Ferulic, and Caffeic acids), flavonoids (β-carotene, α-carotene, β-cryptoxanthin, and Lutein) , Vitamin A, Vitamin-B6 (pyridoxine), Vitamin-C, Vitamin-E, Carbohydrates, Amino acids, Organic acids,  micronutrients (Potassium, Copper), fats (Omega-3 and 6 polyunsaturated fatty acids), dietary fibre and certain volatile compounds. About 25 different types of carotenoids have been isolated from the fruit pulp, which contributes to the colour of the fruit. Phytochemical and nutrient content may vary depending on the cultivar. Mangoes possess potential medicinal properties such as antioxidant, gastro-protective, anti-inflammatory, analgesic, immunomodulatory, anti-microbial, and many more. Mango fruit is an abundant source of all essential nutrients and phytochemicals; it could be ultilized as a nutritional supplement in the prevention and cure of several diseases. A comprehensive report on the nutritional and medicinal properties of fruit is presented below.

Abstract The enormous biological potential of herbal products is one of the main reasons for their frequent use in the production of dietary supplements and functional foods, which, in addition to their nutritional properties, have pharmacological and physiological effects. New scientific knowledge on the isolation of pharmacologically active compounds from complex matrices has led to significant advances in this field. Today, the process of extraction plays a significant scientific role, with “green” technologies occupying a special place in today’s science. Herbal medicine is one of the oldest human skills, which has worn off with its centuries-old application in the path of modern medicine. Microwave-assisted extraction, or more simply, microwave extraction, is a new extraction technique that combines traditional extraction solvents and microwaves. The mentioned method takes less time, consumes less energy, and has strong penetration power into the plant matrix to obtain more oils, but it can also reduce production costs. This can eventually increase the quality of the final product and reduce the product price at the consumer level. Microwave-assisted extraction could be useful to the herbal industry for oil extraction as well as other pharmaceutically important plant components. Based on a comparison and study of published literature, this research examines the present state of extraction procedures. This review includes a detailed discussion of the most important extraction techniques.

Abstract Bael or Aegle marmelos Corrêa is considered a sacred tree by Hindus and is offered to Lord Shiva while worshipping. It grows in the Indian subcontinent and Southeast Asia and is called by various names in different regions. Bael has been used as a traditional medicine in India and other Southeast Asian countries to treat various ailments, including diarrhea, chronic dysentery, constipation, gonorrhea, catarrh, diabetes, deafness, inflammations, ulcerated intestinal mucosa, intermittent fever, melancholia, heart palpitation, and also to control fertility. The ethnomedicinal properties of Bael are owing to its ability to synthesize alkaloids, cardiac glycosides, anthocyanins, flavonoids, steroids, saponins, terpenoids, tannins, lignins, quinones, coumarins, proteins, carbohydrates, amino acids, reducing sugars, fats, and oils. The aegeline, auroptene, umbelliferone, psoralene, marmin, imperatorin, xylorhamnoarabinogalactan I pectic polysaccharide and skimmianine are synthesized by different parts of Bael, and they have shown antibacterial, anti-inflammatory, analgesic, anti-allergic, anthelmintic, antidiabetic, anticancer, cardioprotective and neuroprotective activities in various experimental models. The present review has been written consulting various publications, and different websites including Google Scholar, Pubmed, ScienceDirect, and Google.

Abstract The use of plant viruses as bioherbicides represents a fascinating and promising frontier in modern agriculture and weed management. This review article delves into the multifaceted world of harnessing plant viruses for herbicidal purposes, shedding light on their potential as eco-friendly, sustainable alternatives to traditional chemical herbicides. We begin by exploring the diverse mechanisms through which plant viruses can target and control weeds, from altering gene expression to disrupting essential physiological processes. The article highlights the advantages of utilizing plant viruses, such as their specificity for weed species, minimal impact on non-target plants, and a reduced environmental footprint. Furthermore, we investigate the remarkable versatility of plant viruses, showcasing their adaptability to various weed species and agricultural environments. The review delves into the latest advancements in genetic modification techniques, which enable the engineering of plant viruses for enhanced herbicidal properties and safety. In addition to their efficacy, we discuss the economic and ecological advantages of using plant viruses as bioherbicides, emphasizing their potential to reduce chemical herbicide usage and decrease the development of herbicide-resistant weeds. We also address the regulatory and safety considerations associated with the application of plant viruses in agriculture. Ultimately, this review article underscores the immense potential of plant viruses as bioherbicides and calls for further research, development, and responsible deployment to harness these microscopic agents in the ongoing quest for sustainable and environmentally friendly weed management strategies.

Abstract In higher eukaryotes, the genes’ architecture has become an essential determinant of the variation in the number of transcripts (expression level) and the specificity of gene expression in plant tissue under stress conditions. The modern rise in genome-wide analysis accounts for summarizing the essential factors through the translocation of gene networks in a regulatory manner. Stress tolerance genes are in two groups: structural genes, which code for proteins and enzymes that directly protect cells from stress (such as genes for transporters, osmo-protectants, detoxifying enzymes, etc.), and the genes expressed in regulation and signal transduction (such as transcriptional factors (TFs) and protein kinases). The genetic regulation and protein activity arising from plants’ interaction with minerals and abiotic and biotic stresses utilize high-efficiency molecular profiling. Collecting gene expression data concerning gene regulation in plants towards focus predicts an acceptable model for efficient genomic tools. Thus, this review brings insights into modifying the expression study, providing a valuable source for assisting the involvement of genes in plant growth and metabolism-generating gene databases. The manuscript significantly contributes to understanding gene expression and regulation in plants, particularly under stress conditions. Its insights into stress tolerance mechanisms have substantial implications for crop improvement, making it highly relevant and valuable to the field.

Abstract The rapid pace at which science and technology are developing has made people more skilled at using cutting-edge technologies to increase the value and benefits of horticultural crops. This issue contains the most recent horticultural research findings, which include an intriguing investigation of the factors influencing the properties or growth processes of horticultural crops, methods for controlling and preventing pests and diseases of horticultural crops, the therapeutic and medicinal value of horticultural crops, and techniques for extracting beneficial components from horticultural crops. Reading the articles in this issue may be very beneficial to readers.