The aim of the present study was to determine the effects of single and mixed infections of nematode (Meloidogyne javanica), fungus (Fusarium oxysporum) and bacterium (Xanthomonas axonopodis) on nodulation and pathological parameters of Bambara groundnut (Vigna subterrenea (L.) Verdc.) in field condition. Nematode infested field was used while other pathogens were obtained from diseased plants. The Randomized Complete Block Design (RCBD) was adopted in a 5 × 9 × 5 factorial design (5 blocks, 9 treatments and 5 replicates per treatments) resulting in 225 experimental units. In each experimental unit, three seeds were sown to a depth of 5cm and thinned to one plant per planting hole after germination at day 7. Treatments were inoculated into test plant following standard methods. As a result, the control treatment recorded the highest number of nodules (64.0 ± 6.91), followed by bacterium (45.2 ± 5.11) while N + F + B had the lowest number of root nodules (23.4 ± 2.42). Simultaneous treatment (N + F + B) gave the highest percentage reduction in nodulation (63.44%), followed by treatment N + F7 (56.25%). Fungus treatment recorded the highest mean wilted plants (3.8 + 0.20) followed by N + F7 treatment (3.40 + 0.40). Gall formation in the nematode treatment increased proportionately by 56.33% as the highest recorded, followed by treatment N + F7 with 50.0%. Treatment N + F7 had the highest reproduction factor (Rf) value of 9.30 followed by nematode (8.30), N + B7 (7.40), N + F + B (6.80) and N + F14 (6.50). Zero (0) Rf value was recorded in fungus, bacterium and control treatments. The observed differences in nodulation and pathological parameters among the treatments are significant (P < 0.05). The data provided in this work is important in the control of the three pathogens affecting the productivity of Bambara nut. Formulation of a single protectant should be designed to have potent effects on the three pathogens to achieve effective protection and good production of Bambara nut.

1. Hillocks R, Bennett C, Mponda OM. Bambara nut: A review of utilization, market potential and crop improvement. African Crop Science Journal 2012; 20(1): 1–16.

2. Olayide OE, Donkoh SA, Ansah IGK, et al. Assessing socioeconomic factors influencing production and commercialization of Bambara groundnut as an indigenous climate resilient crop in Nigeria. In: Leal Filho W (editor). Handbook of Climate Change Resilience. Springer Cham; 2018. pp. 1–19. doi: 10.1007/978-3-319-71025-9_158-1.

3. Abejide DR, Falusi OA, Gana AS, et al. Evaluation of seed yield of Nigerian Bambara groundnut [Vigna subterranea (L.) Verdc.] landraces under varying water conditions. Notulae Scientia Biologicae 2018; 10(2): 233–239. doi: 10.25835/nsb10210238.

4. Tlankka NS, Mbega ER, Ndakidemi PA. Potential of indigenous pesticidal plants in the control of field and post-harvest arthropod pests in Bambara groundnuts (Vigna subterranea (L.) Verdc.) in Africa: A Review. American Journal of Plant Research 2020; 11: 745–772. doi: 10.4236/ajps.2020.115054.

5. Bamishaiye OM, Adegbola JA, Bamishaiye E1. Bambara groundnut: An under-utilized nut in Africa. Advances in Agricultural Biotechnology 2011; 1: 60–72.

6. Agyeman K, Asante BO, Berchie JN, et al. Farmers’ perceptions, constraints and preferences for improved Bambara groundnut varieties in Ghana. Journal of Agriculture and Food Research 2021; 3: 100097. doi: 10.1016/j.jafr.2020.100097

7. Iheukwumere CC, Dashiell KE, Mutsaers HJM. Infection complex of soybean mosaic potyvirus SMV-10 and Meloidogyne incognita and effects on the infectivity and multiplication of both pathogens on soybean. International Journal of Nematology 2007; 17(1): 46–54.

8. Al-Hazmi AS, Al-Nadary SN. Interaction between Meloidogyne incognita and Rhizoctonia solani on green beans. Saudi Journal of Biological Sciences 2015; 22(5): 570–574. doi: 10.1016/j.sjbs.2015.04.008

9. Ali WM, Abdel-Mageed MA, Hegazy MGA, et al. Biocontrol agent of root-knot nematode Meloidogyne javanica and root-rot fungi, Fusarium solani in okra morphological, anatomical characteristics and productivity under greenhouse conditions. Scientific Reports 2023; 13: 11103. doi: 10.1038/s41598-023-37837-z

10. Duche TR, Omoigui L, Iheukwumere CC. Variations among Xanthomonas axonopodis pv. vignicola isolates in Benue State, Nigeria. International Journal of Innovation and Scientific Research 2015; 13(1): 271–278.

11. Siddiqui ZA, Fatima M, Alam S. Interactions of Meloidogyne incognita, Xanthomonas campestris, and Rhizobium sp. in the disease complex of chickpea. Turkish Journal of Agriculture and Forestry 2013; 37(2): 173–178. doi: 10.3906/tar-1112-48

12. Jagdale GB, Martin K. Sampling for Plant-Parasitic Nematodes, Identification and Diagnosis. University of Georgia; 2011.

13. Ashley J, Lindse T. Root knot nematode of soybean (soybean disease formation). Available from: https://content.ces.ncsu.edu/root-knot-nematode-of-soybean (accessed on 8 December 2023).

14. Mohit K, Tripathi UK, Ajay T, Singh PKA. Screening of linseed germplasm for resistance/tolerance against Farium oxypersom f sp. Lini (Belley) disease. Journal of Plant Pathology and Microbiology 2014; 5(3): 1000235. doi: 10.4172/2157-7471.1000235

15. Alexopoulos CJ, Mims CW, Blackwell MM. Introductory Mycology, 4th ed. John Wiley and Sons Inc.; 1996. 880p.

16. Ah-You N, Gagnevin L, Grimont PAD, et al. Polyphasic characterization of Xanthomonas pathogenic to members of the Anarcadiaceae and their relatedness to species of Xanthomonas. International Journal of Systemic and Evolutionary Microbiology 2009; 59(2): 306–318. doi: 10.1099/ijs.0.65453-0

17. Iheukwumere CC, Dashiell KE, Mutsaers HJW. Effects of single and combined infection of soybean mosaic virus and Meloidogyne incognita on soybean and replication and pathogenicity of both pathogens. Nematologia Mediterranea 2008; 36: 26–30.

18. Bello TT, Fawole B, Abiodun CC. Susceptibility of seven varieties of pepper and tomato to root-knot nematodes (Meloidogyne spp.) in Ibadan, Nigeria. IOSR Journal of Agriculture and Veterinary Science 2015; 8(1): 79–82. doi: 10.9790/2380-081017982

19. Wonang DL, Akueshi CO. Penetration and development of Meloidogyne incognita and Meloidogyne javanica in twelve different tomato cultivars in Jos, Nigeria. African Journal of Natural Sciences 1998; 2(1): 113–116.

20. Kankam F, Adomako J. Influence of level of root knot nematode (Meloidgyne spp.) on tomato (Solanum lycopersicum L.). Asian Journal of Agriculture and Food Sciences 2014; 2(2).

21. Nene YL, Haware MP, Reddy MV. Chickpea disease resistance—Screening techniques. ICRUISAT; 1981. pp. 1–10.

22. Ismail AE, El-Nagdi WMA, Yasin MY. Histopathology of gall induced chamomile infected with Meloidogyne incognita and Rotylenchus reniformis. Pakistan Journal of Nematology 2004; 22: 143–149.

23. Afolami SO, Atungwu JJ, Odeyemi IS, Orisajo SB. Going beyond gall index in studying and reporting resistance to root-knot nematodes. Nigeria Journal of Plant Protection 2004; 21: 25–32.

24. Okechala OB, Wonang DL. The response of eleven sweet potato (Ipomea batatas (L.) Lam) cultivars to infection by Meloidogyne spp. in Jos, Nigeria. IOSR Journal of Pharmacy and Biological Sciences 2015; 10(4): 42–49. doi: 10.9790/3008-10444249

25. Iheukwumere CC, Orkpeh U. Effect of single and mixed infection of Fusarium oxysporum (Schlect.) Synder and Hansen and Meloidogyne javanica (Treub) Chitwood on growth and yield of soybean (Glycine max (L.) Merr.) Var. TGX 1019-2EN. Nigeran Journal of Botany 2007; 20(2): 397–406.

26. Kolombia YA, Lava Kumar P, Claudius-Cole AO, et al. First report of Meloidogyne enterolobii causing tuber galling damage on white yam (Dioscorea rotundata) in Nigeria. Plant Disease 2016; 100(10): 2173. doi: 10.1094/PDIS-03-16-0348-PDN

27. Zongo E, Néya BJ, Traoré VSE, et al. Impact of Cowpea mottle virus on the growth and yield of Bambara Groundnut (Vigna subterranean (L.) Verdc.). American Journal of Plant Science 2018; 9: 2053–2062. doi: 10.423/ajps.2018.910149

28. Koutsovoulos GD, Poullet M, Elashry A, et al. Genome assembly and annotation of Meloidogyne enterolobii, an emerging parthenogenetic root-knot nematode. Scientific Data 2020; 7(1): 324. doi: 10.1038/s41597-020-00666-0