Infection of mature Pinus densiflora with ectomycorrhizal fungi, Tricholoma matsutake

Myong Ho Choe, Jun Myong Cha, Hui Won Kim, Song Il Pak, Chung Ryol Zhang

Article ID: 3089
Vol 6, Issue 2, 2023

VIEWS - 177 (Abstract) 125 (PDF)

Abstract


We carried out research work to infect the roots of mature pine (Pinus densiflora) with Tricholoma matsutake for cultivation of the pine mushroom. Four-month-old and one-year-old seedlings and forty-year-old mature pine were treated with α-NAA (α-naphthalene acetic acid) as rooting agent for generating new rootlets. The optimum concentrations of α-NAA for generating new rootlets in four-month-old and one-year-old pine seedlings were 0.5 mg per root, at which numbers of generated new rootlets were approximately 2–3 times higher than in control. The mature pine treated with 1.0 mg of α-NAA per root produced approximately 1.7 times more new rootlets than untreated. Roots in 15 mature pines were treated with α-NAA, and about 79% of the treated roots successfully generated new branching roots. For mycorrhizal synthesis, the new rootlets without contamination were inoculated with mycelia of matsutake cultured in a glass container with sterilized vermiculite substrate. After 4 months, it was identified by ITS specific primer method that about 50% of the analyzed root samples were infected with matsutake. The results showed that the roots of mature pine can be infected by matsutake.

Keywords


Tricholoma matsutake; ectomycorrhizal fungi; α-NAA; Pinus densiflora; symbiosis

Full Text:

PDF


References


1. Bergius N, Danell E. The Swedish matsutake (Tricholoma nauseosum syn. T. matsutake): Distribution, abundance and ecology. Scandinavian Journal of Forest Research 2000; 15(3): 318–325. doi: 10.1080/028275800447940

2. Yamada A, Kobayashi H, Murata H, et al. In vitro ectomycorrhizal specificity between the Asian red pine Pinus densiflora and Tricholoma matsutake and allied species from worldwide Pinaceae and Fagaceae forests. Mycorrhiza 2010; 20: 333–339. doi: 10.1007/s00572-009-0286-6

3. Hosford D, Pilz D, Molina R, Amaranthus M. Ecology and Management of the Commercially Harvested American Matsutake Mushroom. DIANE Publishing; 1998.

4. Ka KH, Kim HS, Hur TC, et al. Analysis of environment and production of Tricholoma matsutake in matsutake-infected pine trees. The Korean Journal of Mycology 2018; 46(1): 34–42. doi: 10.4489/KJM.20180005

5. Lee WH, Han SK, Kim BS, et al. Proliferation of Tricholoma matsutake mycelial mats in pine forest using mass liquid inoculum. Mycobiology 2007; 35(2): 54–61. doi: 10.4489/MYCO.2007.35.2.054

6. Peter M. Ectomycorrhizal fungi–fairy rings and the wood‐wide web. New Phytologist 2006; 171(4): 685–687. doi: 10.1111/j.1469-8137.2006.01856.x

7. Reed JW. Roles and activities of Aux/IAA proteins in Arabidopsis. Trends in Plant Science 2001; 6(9): 420–425. doi: 10.1016/S1360-1385(01)02042-8

8. Sudadi S, Suryono S. Exogenous application of tryptophan and indole acetic acid (IAA) to induce root nodule formation and increase soybean yield in acid, neutral and alkaline soil. AGRIVITA, Journal of Agricultural Science 2015; 37(1): 37–44. doi: 10.17503/agrivita.v37i1.444

9. Mustafa A, Hussain A, Naveed M, et al. Response of okra (Abelmoschus esculentus L.) to soil and foliar applied L-tryptophan. Soil & Environment 2016; 35(1): 76–84.

10. Gay G, Normand L, Marmeisse R, et al. Auxin overproducer mutants of Hebeloma cylindrosporum Romagnesi have increased mycorrhizal activity. New Phytologist 1994; 128(4): 645–657. doi: 10.1111/j.1469-8137.1994.tb04029.x

11. Podila GK. Signaling in mycorrhizal symbioses: Elegant mutants lead the way. New Phytologist 2002; 154(3): 541–545.

12. Krause K, Henke C, Asiimwe T, et al. Biosynthesis and secretion of indole-3-acetic acid and its morphological effects on Tricholoma vaccinum-spruce ectomycorrhiza. Applied and Environmental Microbiology 2015; 81(20): 7003–7011. doi: 10.1128/AEM.01991-15

13. Wang Y. Tricholoma Matsutake [PhD thesis]. University of Otago; 1995.

14. Vaario LM, Guerin-Laguette A, Gill WM, et al. Only two weeks are required for Tricholoma matsutake to differentiate ectomycorrhizal Hartig net structures in roots of Pinus densiflora seedlings cultivated on artificial substrate. Journal of Forest Research 2000; 5(4): 293–297. doi: 10.1007/BF02767125

15. Guerin-Laguette A, Matsushita N, Lapeyrie F, et al. Successful inoculation of mature pine with Tricholoma matsutake. Mycorrhiza 2005; 15: 301–305. doi: 10.1007/s00572-005-0355-4

16. Rincón A, Ruiz-Díez B, García-Fraile S, et al. Colonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus. FEMS Microbiology Ecology 2005; 51(3): 303–311. doi: 10.1016/j.femsec.2004.09.006

17. Kikuchi K, Matsushita N, Guerin-Laguette A, et al. Detection of Tricholoma matsutake by specific ITS primers. Mycological Research 2000; 104(12): 1427–1430. doi: 10.1017/S0953756200002653

18. Mathur N, Vyas A. Changes in isozyme patterns of peroxidase and polyphenol oxidase by VAM fungi in roots of Ziziphus species. Journal of Plant Physiology 1995; 145(4): 498–500. doi: 10.1016/S0176-1617(11)81777-3

19. Bending GD, Read DJ. Effects of the soluble polyphenol tannic acid on the activities of ericoid and ectomycorrhizal fungi. Soil Biology and Biochemistry 1996; 28(12): 1595–1602. doi: 10.1016/S0038-0717(96)00257-X

20. Yun W, Hall IR, Evans LA. Ectomycorrhizal fungi with edible fruiting bodies 1. Tricholoma matsutake and related fungi. Economic Botany 1997; 51(3): 311–327.

21. Hall IR, Yun W, Amicucci A. Cultivation of edible ectomycorrhizal mushrooms. Trends in Biotechnology 2003; 21(10): 433–438. doi: 10.1016/S0167-7799(03)00204-X

22. Guerin-Laguette A, Shindo K, Matsushita N, et al. The mycorrhizal fungus Tricholoma matsutake stimulates Pinus densiflora seedling growth in vitro. Mycorrhiza 2004; 14: 397–400. doi: 10.1007/s00572-004-0322-5

23. Suzuki K. Ectomycorrhizal ecophysiology and the puzzle of Tricholoma matsutake. Journal of the Japanese Forest Society (Japan) 2005; 87(1): 90–102.




DOI: https://doi.org/10.24294/th.v6i2.3089

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This site is licensed under a Creative Commons Attribution 4.0 International License.