Abstract Studies to evaluate the response of passion fruit seedlings in terms of emergence, nursery, and early field growth to growing media and mulching were carried out at the Teaching and Research Farm of Joseph Sarwuan Tarka University Makurdi between July and December 2018. Treatments consisted of five media, composted from readily available substrates. The five nursery media were; medium 1:1:2:3 (SB) composed of top soil + poultry manure + river sand; medium 2:1:2:3 (RHB) – rice hull + poultry manure + river sand; medium 3:2:3:1 (RHB) – rice hull + poultry manure + river sand; medium 4:1:4:3 (SDB) – sawdust + poultry manure + river sand and medium 5:1:2:3 (SDB) – sawdust + poultry manure + river sand. For the nursery experiment, treatments were the five potting media, while the field trial was a 5 × 2 factorial arrangement consisting of the five growing media and mulching status (mulch and no mulch). In both cases, treatments were laid out in randomized designs that were replicated three times. Results showed that there were no significant differences in all the emergence traits evaluated. However, medium M5 (sawdust based) showed superior performance in most of the seedling characters evaluated. Under field conditions, the sawdust based media (M4 and M5) gave the best growth of passion fruit seedlings compared to the other potting media. Application of mulch, however, did not elicit any significant response in plant growth. It is therefore conclusive that sawdust based growing media could be used to produce high quality passion fruit seedlings with the prospect of excellent performance under field conditions.

Abstract An experiment was carried out to investigate the effect of different organic nutrient solutions and day of harvest on growth parameters, biomass and chemical composition of hydroponically grown sorghum red fodder. The experiment was a 3 × 2 factorial design comprising of 3 nutrient solutions (cattle, poultry and rabbit) and 2 harvesting regimes (8th and 10th day). Cattle, poultry and rabbit dungs were collected fresh and processed into nutrient solutions. Sorghum red seeds were treated, planted on trays, and irrigated twice per day with organic nutrient solution according to the treatments. Growth parameters which were investigated included fodder mat thickness, seedling height, leaf length and width, number of leaves, fresh and dry matter yield; and proximate composition. The results showed that sorghum red fodder irrigated with cattle manure nutrient solution (NS) harvested at 10 days was higher in all, except one (fodder mat thickness) of the growth parameters considered. The crude protein (CP) was highest and similar ( P > 0.05) for Poultry NS harvested at 8 and 10 days, and Cattle NS at 10 days (13.13%, 12.67%, and 12.69% respectively). The ash content also favored Cattle NS at 10 days. Cattle NS at 10 days harvest was significantly ( P < 0.05) the highest (7.00%), but comparable ( P > 0.05) with Rabbit NS at 10 days for NDF. Fresh and DM yields were highest for Cattle harvested at 10 and 8 days respectively. The study recommends Cattle NS as hydroponic organic NS for sorghum red as it enhances fresh and dry matter yields, and nutritive values.

Abstract A reservoir of vegetation, wildlife, and medicinal plant abundance is represented by the Haridwar forest divisions. This study deals with the results of ethnobotanical survey of medicinal plants conducted in the Haridwar forest division during the period of December 2016 and March 2019. The information on folk medicinal use of plants were gathered by interviewing with local healers and Vaidya’s who have long been advising the folk medicines for medication of various disorders. The important folk medicinal data of 33 medicinal plants species belonging to 22 families and 33 genera practiced by tribal and local people of the study area has been recorded by the survey team of the Institute. Fabaceae followed by the Lamiacea and Asteraceae were the dominant families. The species diversity showed maximum exploration of Trees, Herbs followed by Shrubs and Climbers. Leaves, seed and root were the most prevalently used part in study followed by the stem bark, fruit, flower, stem and fruit pulp. During the study it was observed that the traditional practices of Gujjars of Uttarakhand have close relation with forests and have strong dependency on the same for food, medicine, timber and fodder etc. The information recorded for the treatment in different ailments has been presented in the paper in the pie charts and tabular form. In the recorded information most of the plants along with Plant name, Family name, Voucher Specimen No., Local Name/Unani name, Part Used, Diseases/Condition and Habitat/ICBN status so as to enrich the existing knowledge on ethnopharmacology. Many of the medications used today have their roots in traditional knowledge of medicinal plants and indigenous uses of plant material, and there are still a plethora of potentially useful pharmaceutical chemicals to be found. In this regard, more in-depth field research could aid in the discovery of novel plant species utilized in indigenous medical systems to improve patient needs. With this aim this study was conducted to explore and trace the ethnobotanical potential of flora of the Haridwar forest division so that it could prove to be immensely advantageous for both the development of new medications to treat dreadful and catastrophic illnesses as well as for the study and preservation of cultural and social variety.

Abstract Horticultural crops are rich in constituents such as proteins, carbohydrates, vitamins, and minerals important for human health. Under biotic and abiotic stress conditions, rhizospheric bacteria are powerful sources of phytohormones such as indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and Plant growth regulators including cytokines, ammonia, nitrogen, siderophores, phosphate, and extra cellular enzymes. These phytohormones help horticultural crops grow both directly and indirectly. In recent agricultural practices, the massive use of chemical fertilizers causes a major loss of agricultural land that can be resolved by using the potent plant growth-promoting rhizospheric bacteria that protect the agricultural and horticultural crops from the adverse effect of phytopathogens and increase crop quality and yield. This review highlights the role of multifunctional rhizospheric bacteria in the growth promotion of horticultural crops in greenhouse conditions and agricultural fields. The relevance of plant growth hormones in horticultural crops highlighted in the current study is crucial for sustainable agriculture.

Abstract This study aims to determine the effects of monosodium glutamate (MSG) dosage on the yield of long beans ( Vigna sinensis L.) of the Peleton variety. The use of MSG as a food ingredient has been a topic of debate, but research on its impact on plant growth is still limited, especially regarding long beans. Therefore, this research is important for providing further understanding of the influence of MSG on long beans plants. The study was conducted from July to October 2023 in Mata Air Village, Central Kupang District, Kupang Regency, East Nusa Tenggara Province. The research method used was a Randomized Complete Block Design (RCBD) with 9 treatments and 3 replications. The treatments included: No MSG, MSG at doses of 2.5 g/plant, 5 g/plant, 7.5 g/plant, 10 g/plant, 12.5 g/plant, 15 g/plant, 17.5 g/plant, and 20 g/plant. Parameters observed included flowering age (days), number of pods (pieces), pod length (cm), and pod weight (g). Based on the results and discussion, it can be concluded that MSG application had a significant effect on the number, length, and weight of pods, but had a non-significant effect on flowering age. The treatment of 15 g/plant was identified as the optimal MSG dosage for the plants, resulting in the highest number of pods (16.2), longest pod length (60.4 cm), and highest pod weight (256.4 g/plant). This research is innovative in exploring the potential use of monosodium glutamate (MSG) on long beans plants, particularly the Peleton variety. The focus on MSG application as a growth stimulant is an innovative step that has been less studied previously. The discovery of the optimal MSG dosage (15 g/plant) for achieving the best results provides valuable information for farmers to enhance productivity efficiently, sustainably, and environmentally friendly. Information about MSG’s potential as a plant stimulant can serve as a starting point for more sustainable agricultural strategies aimed at optimizing available resources.

Abstract In this investigation the effect of collection seasons of explants (winter, spring and summer), type of explants (leaf disc and intermodal segments) and length of explants (0.5, 1.0 and 1.5 cm) for callusing in low-chill peach were standardized. The maximum callus induction (97.78%) in the low-chill peach was obtained from the intermodal segments of 0.5 cm in length used as an explant collected during spring season. The structural changes on the surface of the callus (5–7 weeks old yellowish green compact callus) during the progress of somatic embryogenesis of low-chill peach from the both intermodal segment as well as leaf disc derived callus were also examined with the use of scanning electron microscope (SEM). The SEM studies indicated that callus derived from internodal segment explant had the highest frequency of somatic embryos than callus from leaf discs. The SEM investigation, also demonstrated the sequential events/steps leading to low-chill peach somatic embryogenesis which was originating from somatic embryo mother cells through one unicellular pathway. Two types of calli were morphologically distinguished in both leaf disc and intermodal segment generated callus and these were the compact, well organized yellowish green embryogenic callus, containing large number of small, rich cytoplasmic, starch containing meristematic cells and soft and unorganized non-embryogenic callus containing sparsely cytoplasmic, vacuolated, and large cells devoid of metabolic reserves. The present SEM studies clearly demonstrated that somatic cells from peach explants generated callus could develop into fully differentiated somatic embryos through the characteristic embryological patterns of differentiation.

Abstract The WRKY gene family plays a very diverse role in plant growth and development. These genes contained an evolutionarily conserved WRKY DNA binding domain, which shows functional diversity and extensive expansion of the gene family. In this study, we conducted a genome-wide comparative analysis to investigate the evolutionary aspects of the WRKY gene family across various plant species and revealed significant expansion and diversification ranging from aquatic green algae to terrestrial plants. Phylogeny reconstruction of WRKY genes was performed using the Maximum Likelihood (ML) method; the genes were grouped into seven different clades and further classified into algae, bryophytes, pteridophytes, dicotyledons, and monocotyledons subgroups. Furthermore, duplication analysis showed that the increase in the number of WRKY genes in higher plant species was primarily due to tandem and segmental duplication under purifying selection. In addition, the selection pressures of different subfamilies of the WRKY gene were investigated using different strategies (classical and Bayesian maximum likelihood methods (Data monkey/PAML)). The average dN/dS for each group are less than one, indicating purifying selection. Our comparative genomic analysis provides the basis for future functional analysis, understanding the role of gene duplication in gene family expansion, and selection pressure analysis.

Abstract The experiments were carried out to validate an analytical method and to examine the impact of various decontaminating solutions on the removal of acephate residues from okra. Acephate analysis was performed using HPLC-UV, and sample extraction was done using the QuEChERS method. Method validation encompassed assessing specificity, linearity, precision, accuracy, as well as limits of detection (LOD) and quantification (LOQ). The method exhibited excellent linearity with R 2 values ≥ 0.99. LOD and LOQ were determined at 0.5 µg mL −1 and 2 µg mL −1 , respectively. The results indicated average recoveries ranging from 80.2% to 83.3% with a % RSD below 5%. The decontamination procedures include rinsing with running tap water, soaking in lukewarm water, 2% CH 3 COOH, 1% NaCl, 5% NaHCO 3 , 0.01% KMnO 4 , and in commercially available decontamination products such as nimwash, veggie clean, and arka herbiwash for a duration 10 minutes. Among all the treatments, soaking in nimwash solution showed remarkable effectiveness (96.75% removal), followed by veggie clean (94.97% removal) and arka herbiwash (95.80% removal). Washing okra samples in running tap water was found to be the least effective compared to other treatments.

Abstract Soil salinity is a major abiotic stress that drastically hinders plant growth and development, resulting in lower crop yields and productivity. As one of the most consumed vegetables worldwide, tomato ( Solanum lycropersicum L.) plays a key role in the human diet. The current study aimed to explore the differential tolerance level of two tomato varieties (Rio Grande and Agata) to salt stress. To this end, various growth, physiological and biochemical attributes were assessed after two weeks of 100 mM NaCl treatment. Obtained findings indicated that, although the effects of salt stress included noticeable reductions in shoots’ and roots’ dry weights and relative growth rate as well as total leaf area, for the both cultivars, Rio Grande performed better compared to Agata variety. Furthermore, despite the exposure to salt stress, Rio Grande was able to maintain an adequate tissue hydration and a high leaf mass per area (LMA) through the accumulation of proline. However, relative water content, LMA and proline content were noticeably decreased for Agata cultivar. Likewise, total leaf chlorophyll, soluble proteins and total carbohydrates were significantly decreased; whereas, malondialdehyde was significantly accumulated in response to salt stress for the both cultivars. Moreover, such negative effects were remarkably more pronounced for Agata relative to Rio Grande cultivar. Overall, the current study provided evidence that, at the early growth stage, Rio Grande is more tolerant to salt stress than Agata variety. Therefore, Rio Grande variety may constitute a good candidate for inclusion in tomato breeding programs for salt-tolerance and is highly recommended for tomato growers, particularly in salt-affected fields.

Abstract Medicinal herbs have been extensively utilized in the remediation of various health conditions. Dialium guineense fruit pulp, also well known as Velvet Tamarind is widely consumed in West Africa for its dietary and medicinal properties. The study aims to analyze the phytochemical constituents, vitamin content and the in vitro antioxidant effect of Dialium guineense fruit pulp (DGFP). The phytochemical constituents, vitamins (C, E, B1-12) composition, and in vitro antioxidant activity were examined utilizing standardized analytical methods. The qualitative and quantitative phytochemical screening of the fruit pulp of Dialium guineense was also carried out; the result indicated the presence of flavonoids, alkaloids, saponins, tannins, terpenoids, phenols, steroids, and cardiac glycosides in varying concentrations. The vitamin composition revealed that vitamin C was higher than other vitamins in the fruit pulp. The DPPH (2,2-diphenyl-1-picrylhydrazyl) and nitric oxide scavenging assay showed high radical scavenging activity while the FRAP (Ferric reducing antioxidant power) assay revealed significant reducing power. This indicates that Dialium guineense fruit pulp has potential health benefits.

Abstract Banana ( Musa spp.) productivity is limited by sodic soils, which impairs root growth and nutrient uptake. Analyzing root traits under stress conditions can aid in identifying tolerant genotypes. This study investigates the root morphological traits of banana cultivars under sodic soil stress conditions using Rhizovision software. The pot culture experiment was laid out in a Completely Randomized Design (CRD) under open field conditions, with treatments comprising the following varieties: Poovan (AAB), Udhayam (ABB), Karpooravalli (ABB), CO 3 (ABB), Kaveri Saba (ABB), Kaveri Kalki (ABB), Kaveri Haritha (ABB), Monthan (ABB), Nendran (AAB), and Rasthali (AAB), each replicated thrice. Parameters such as the number of roots, root tips, diameter, surface area, perimeter, and volume were assessed to evaluate the performance of different cultivars. The findings reveal that Karpooravali and Udhayam cultivars exhibited superior performance in terms of root morphology compared to other cultivars under sodic soil stress. These cultivars displayed increased root proliferation, elongation, and surface area, indicating their resilience to sodic soil stress. The utilization of Rhizovision software facilitated precise measurement and analysis of root traits, providing valuable insights into the adaptation mechanisms of banana cultivars to adverse soil conditions.

Abstract Plant growth-promoting rhizobacteria (PGPR) offer eco-friendly alternatives to chemical fertilizers, promoting sustainable agriculture by enhancing soil fertility, reducing pathogens, and aiding in stress resistance. In agriculture, they play a crucial role in plant growth promotion through the production of agroactive compounds and extracellular enzymes to promote plant health and protection against phytopathogens. In the rhizosphere, diverse microbial interactions, including those with bacteria and fungi, influence plant health by production of antimicrobial compounds. The antagonism displayed by rhizobacteria plays a crucial role in shaping microbial communities and has potential applications in developing a natural and environmentally friendly approach to pest control. The rhizospheric microbes showcase their ecological importance and potential for biotechnological applications in the context of plant-microbe interactions. The extracellular enzymes produced by rhizospheric microbes like amylases, chitinases, glucanases, cellulases, proteases, and ACC deaminase contribute to plant processes and stress response emphasizing their importance in sustainable agriculture. Moreover, this review highlights the new paradigm including artificial intelligence (AI) in sustainable horticulture and agriculture as a harmonious interaction between ecological networks for promoting soil health and microbial diversity that leads to a more robust and self-regulating agricultural system for protecting the environment in the future. Overall, this review emphasizes microbial interactions and the role of rhizospheric microbial extracellular enzymes which is crucial for developing eco-friendly approaches to enhance crop production and soil health.

Abstract Our study evaluated the effect of vanadium (V) on the behavior of Zinnia elegans “double variegated”. In this experiment, Zinnia plants grown in a greenhouse were fed with a nutrient solution and two concentrations of vanadium (0, 6, and 10 μm) applied four times during the experiment. The V at its levels of 6 µm and 10 µm increased plant length, number of inflorescences and fresh weight. We observed that during the development and appearance of flower buds, and flowering were earlier with the addition of 6 µm and 10 µm. During harvest the changes in size and shape were homogeneous with the control treatment. With the addition of 6 µm, flowers of different sizes were induced, with non-uniform petals, but with different shades of color. With 10 µm the shape of the petals, the distance between them and changes in the shades of the flowers were modified. The postharvest life for the flowers of the control treatment was shorter (15 days), the petals, anthers and floral disc at this time were observed in a poor condition. While 6 µm and 10 µm had a longer postharvest life (20 days), the flowers had a good presentation, their colors were more intense compared to the harvest stage. The application of this beneficial element contributed to the development and flowering of Zinnia in the greenhouse. It is suggested that future research be carried out on the accumulation and/or concentration of vanadium in the different stages of growth or its effect on the concentration of other nutrients.