Study on the preparation and antibacterial properties of CTAB-coated AuNPs

Yayun Ma, Mei Liu, Jiao Li, Xuanyi Li, Zongqi Yang

Article ID: 1407
Vol 5, Issue 1, 2022

VIEWS - 445 (Abstract) 325 (PDF)

Abstract


In this paper, spherical gold nanoparticles (AuNPs), rod-shape AuNPs and triangular AuNPs were synthesized using CTAB as the coating reagent, and their bactericidal properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were studied. By the plate count method and turbidity method, the minimum bactericidal concentrations (MBC) and the minimum bacteriostasis concentrations (MIC) to the two kinds of bacteria were determined. The MIC of rod-shape AuNPs, triangular AuNPs and spherical AuNPs to E. coli were 0.65 μg/mL, 3.71 μg/mL, 21.21 μg/mL, and MBC were 1.30 μg/mL, 11.09 μg/mL, 21.21 μg/mL, respectively. The MIC to S. aureus were 0.26 μg/mL, 0.56 μg/mL, 2.65 μg/mL, while MBC were 0.52 μg/mL, 1.11 μg/mL, 2.65 μg/mL, respectively. The results showed that the bactericidal effect of rod-shape AuNPs on E. coli and S. aureus was higher than that of the other two forms, and the bactericidal effect of three different forms of AuNPs on S. aureus was better than that on E. coli.

Keywords


AuNPs; Different Forms; S. aureus; E. coli; Antibacterial Properties

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References


1. Dizaj SM, Lotfipour F, Barzegar-Jalali M, et al. Antimicrobial activity of the metals and metal oxide nanoparticle. Materials Science and Engineering: C 2014; 44: 278–284.

2. Ma W, Cui Y, Zhao Y, et al. Progress of antibacterial mechanisms study on nanoparticles. Acta Biophysica Sinica 2010; 26(8): 638–648.

3. Zhao Y, Tian Y, Cui Y, et al. Small molecule-capped gold nanoparticles as potent antibacterial agents that target gram-negative bacteria. Journal of the American Chemical Society 2010; 132(35): 12349–12356.

4. Li Y, Chen X. Preparation and mechanism of graphene-Ag antibacterial materials. Journal of Liaocheng University (Natural Science Edition) 2014; 27(3): 71–74.

5. Corma A, Garcia H. Supported gold nanoparticles as catalysts for organic reactions. Chemical Society Reviews 2008; 37: 2096–2126.

6. Prabaharan M, Grailer JJ, Pilla S, et al. Gold nanoparticles with a monolayer of doxorubicin-conjugated amphiphilic block copolymer for tumor-targeted drug delivery. Biomaterials 2009; 30(30): 6065–6075.

7. Yáñez-Sedeño P, Pingarrón JM. Gold nanoparticle-based electrochemical biosensors. Analytical and Bioanalytical Chemistry 2005; 382(4): 884–886.

8. Lin YW, Huang CC, Chang HT. Gold nanoparticle probes for the detection of mercury, lead and copper ions. Analyst 2011; 136(5): 863–871.

9. Guo Y, Wang Z, Qu W, et al. Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles. Biosensors and Bioelectronics 2011; 10(15): 4064–4069.

10. Gu H, Ho PL, Tong E, et al. Presenting vancomycin on nanoparticles to enhance antimicrobial activities. Nano Letters 2003; 3(9): 1261–1263.

11. Tom RT, Suryanarayanan V, Reddy PG, et al. Ciprofloxacin-protected gold nanoparticles. Langmuir 2004; 20(5): 1909–1914.

12. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Applied and Environmental Microbiology 2020; 73(6): 1712–1720.

13. Penders J, Stolzoff M, Hickey DJ, et al. Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles. International Journal of Nanomedicine 2017; 12: 2457–2468.

14. Yang X, Yang M, Pang B, et al. Gold nanomaterials at work in biomedicine. Chemical Reviews 2015; 115(19): 10410–10488.

15. Jana NR, Gearheart L, Murphy CJ. Seeding growth for size control of 5−40 nm diameter gold nanoparticles. Langmuir 2001; 17(22): 6782–6786.

16. Wang Y, Zhou X, Xu C, et al. Gold nanorods as visual sensing platform for chiral recognition with naked eyes. Scientific Reports 2018; 8(1): 5296–5304.

17. Guo Z, Fan X, Liu L, et al. Achieving high-purity colloidal gold nanoprisms and their application as biosensing platforms. Journal of Colloid and Interface Science 2010; 348(1): 29–36.

18. Fang M, Chen J, Xu X, et al. Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests. International Journal of Antimicrobial Agents 2006; 27(6): 513–517.

19. Kim J, Marshall MR, Wei CI. Antibacterial activity of some essential oil components against five foodborne pathogens. Journal of Agricultural and Food Chemistry 1995; 43(11): 2839–2845.

20. Cui Y, Zhao Y, Tian Y, et al. The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli. Biomaterials 2012; 33(7): 2327–2333.

21. Zhang P, Li B, Du J, et al. Gold nanoparticles coated by polyethylenimine-g-bovine serum albumin with different morphologies for effective gene delivery. Journal of Controlled Release 2017; 259: e102–e103.

22. Seil JT, Webster TJ. Antimicrobial applications of nanotechnology: Methods and literature. International Journal of Nanomedicine 2012; 7(1): 2767–2781.

23. Simon-Deckers A, Loo S, Mayne-L’hermite M, et al. Size-, composition- and shape-dependent toxicological impact of metal oxide nanoparticles and carbon nanotubes toward bacteria. Environmental Science & Technology 2009; 43(21): 8423–8429.

24. Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harbor Perspectives in Biology 2010; 2(5): a000414.

25. Dickson JS, Koohmaraie M. Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces. Applied and Environmental Microbiology 1989; 55(4): 832–836.




DOI: https://doi.org/10.24294/can.v5i1.1407

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