Kinnow production is hampered due to the lack of micronutrient applications such as zinc (Zn), iron (Fe), and manganese (Mn), which play a significant role in the metabolic activities of the plant, affecting yield and quality. The farmers of the region use mineral micronutrient fertilizers, but it leads to phytotoxicity due to unoptimized fertilizer application dose. In the present investigation, an attempt has been made to optimize the Zn, Mn, and Fe minerals dose as tank mix foliar application for improvement of fruit yield, quality, and uptake of nutrients. The twelve combinations of different doses of zinc sulphate, manganese sulphate, and ferrous sulphate fertilizers replicated three times were tested at kinnow orchards established at Krishi Vigyan Kendra, Bathinda, Punjab, India. The data revealed that the fruit drop was significantly low in the treatment F₁₂ (43.4%) (tank mix spray of 0.3% ZnSO₄ + 0.2% MnSO₄ + 0.1% FeSO₄ ) compared to control treatment. The fruit yield per tree was significantly higher in the treatment F12 compared to untreated control. The juice percentage was also recorded higher in treatment F12 as compared to control, and the juice percentage improved by 2.6%. The leaf nutrient analysis also revealed translocation of higher amount of nutrient from leaf to fruit under optimized supply of micronutrient. Thus, the application of tank mix spray of 0.3% ZnSO₄ + 0.2% MnSO₄ + 0.1% FeSO₄ may be used for better fruit yield and quality.

1. Radović A, Nikolić D, Milatović D, et al. The effect of plant hormones on pollen germination and pollen tube growth of almond cultivars. Acta Horticulturae 2016; 1139: 375–380. doi: 10.17660/actahortic.2016.1139.65

2. Graham RD, Webb MJ. Micronutrients and disease resistance and tolerance in plants. Micronutrients in Agriculture 1991; 4: 329–370. doi: 10.2136/sssabookser4.2ed.c10

3. Buchanan BB, Gruissen W, Jones RL. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists; 2000.

4. Marschner P. Marschner’s Mineral Nutrition of Higher Plants, 3rd ed. Elsevier Academic Press. 2011.

5. Reynolds MP, Braun HJ (editors). Wheat Improvement. Springer International Publishing; 2022. doi: 10.1007/978-3-030-90673-3

6. Dimkpa CO, Bindraban PS. Fortification of micronutrients for efficient agronomic production: A review. Agronomy for Sustainable Development 2016; 36(1). doi: 10.1007/s13593-015-0346-6

7. Sillanpää M. Micronutrients assessment at the country level: An international study. FAO Soils Bulletin 1990; 63: 208.

8. Rai S, Singh PK, Mankotia S, et al. Iron homeostasis in plants and its crosstalk with copper, zinc, and manganese. Plant Stress 2021; 1: 100008. doi: 10.1016/j.stress.2021.100008

9. Havlin JL. Soil Fertility and Fertilizers: An Introduction to Nutrient Management, 7th ed. Prentice Hall; 2004.

10. Hafez OM, El-Metwally IM. Efficiency of zinc and potassium sprays alone or in combination with some weed control treatments on weeds growth, yield and fruit quality of Washington navel orange orchards. Journal of Applied Sciences Research 2007; 3(7): 613–621.

11. Tariq M, Sharif M, Shah Z, et al. Effect of foliar application of micronutrients on the yield and quality of sweet orange (Citrus sinensis L.). Pakistan Journal of Biological Sciences 2007; 10(11): 1823–1828. doi: 10.3923/pjbs.2007.1823.1828

12. Barooah A, Chakravarty M, Hazarika B. Response of nutrient management on growth, yield and quality of citrus crop: A review. Journal of Pharmacognosy and Phytochemistry 2022; 11(5): 291–297.

13. Sajida P. Effect of foliar application of zinc, manganese and boron in combination with urea on the yield of sweet orange. Pakistan Journal of Agricultural Research 2000; 16(2): 135–141.

14. Yaseen M, Nadeem M, Hussain S. Investigating the effectiveness of micropower foliar spray on growth and yield of different crops. Pakistan Journal of Life and Social Sciences 2004; 2(2): 156–158.

15. Alva AK, Paramasivam S, Obreza TA, et al. Nitrogen best management practice for citrus trees. Scientia Horticulturae 2006; 107(3): 233–244. doi: 10.1016/j.scienta.2005.05.017

16. Qamar-uz-Zaman, Schumann AW. Nutrient management zones for citrus based on variation in soil properties and tree performance. Precision Agriculture 2006; 7(1): 45–63. doi: 10.1007/s11119-005-6789-z

17. Central Ground Water Board. Play on artificial recharger to ground water and water conservation in overexploration blocks of Bathinda District, Punjab. Available online: http://cgwb.gov.in/District_Profile/Punjab/Bathinda.pdf (assessed on 21 December 2023).

18. Piper CS. Soil and Plant Analysis. Pp. 368. Hans Publishers; 1996.

19. Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science 1934; 37(1): 29–38. doi: 10.1097/00010694-193401000-00003

20. Puri AH. Soil, Their Physics and Chemistry. Reinhold Publications Corporation; 1950.

21. Olsen SR, Cole CV, Watanabe FS, Dean LA. Estimation of available phosphorus by extraction with sodium biocarbonate. Available online: https://ia903207.us.archive.org/21/items/estimationofavai939olse/estimationofavai939olse.pdf (accessed on 21 December 2023).

22. Jackson ML. Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd.; 1973. 498p.

23. Merwin HD, Peech M. Exchangeability of soil potassium in the sand, silt, and clay fractions as influenced by the nature of the complementary exchangeable cation. Soil Science Society of America Journal 1951; 15: 125–128. doi: 10.2136/sssaj1951.036159950015000c0026x

24. Subbaiah BV. A rapid procedure for estimation of available nitrogen in soil. Current Science 1956; 25: 259–260.

25. Thind SK. Package of practices for cultivation of fruits. Available online: https://www.pau.edu/content/ccil/pf/pp_fruits.pdf (accessed on 21 December 2023).

26. Isaac RA, Johnson WC. Determination of total nitrogen in plant tissue, using a block digestor. Journal of the Association of Official Analytical Chemists 1976; 59(1): 98–100. doi: 10.1093/jaoac/59.1.98

27. Chapman HD, Pratt PF. Methods of analysis for soils, plants and waters. Soil Science 1962; 93(1): 68. doi: 10.1097/00010694-196201000-00015

28. Miller RH, Keeney DR. Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy (ASA) and Soil Science Society of America; 1982.

29. Liaquat M, Ali I, Ahmad S, et al. Efficiency of exogenous zinc sulfate application reduced fruit drop and improved antioxidant activity of ‘kinnow’ mandarin fruit. Brazilian Journal of Biology 2023; 83. doi: 10.1590/1519-6984.244593

30. Lindsay WL, Norvell WA. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal 1978; 42(3): 421–428. doi: 10.2136/sssaj1978.03615995004200030009x

31. Shapiro SS, Wilk MB. An analysis of variance test for normality (complete samples). Biometrika 1965; 52(3–4): 591–611. doi: 10.1093/biomet/52.3-4.591

32. Zoremtluangi J, Saipari E, Mandal D. Influence of foliar micronutrients on growth, yield and quality of Khasi mandarin (Citrus reticulata Blanco) in Mizoram. Research on Crops 2019; 20(2): 322–327. doi: 10.31830/2348-7542.2019.047

33. Marschner H. Mineral Nutrition of Higher Plant. Academic Press; 1995. pp. 330–355.

34. Nawaz MA, Ahmad W, Ahmad S, Khan MM. Role of growth regulators on preharvest fruit drop, yield and quality in Kinnow mandarin. Pakistan Journal of Botany 2008; 40(5): 1971–1981.

35. Akula V, Delvadia DV, Sharma LK, et al. Effect of micronutrient application on flowering, fruiting and yield of acid lime (Citrus aurantifolia L.). cv. Kagzi lime. International Journal of Tropical Agriculture 2014; 32(3/4): 331–334.

36. Goren R, Huberman M, Zhong GY. Basics aspects of hormonal control of abscission. Proceedings International Society of Citriculture 2000; 9: 591–594.

37. Singh Y, Thakur N, Meena NK. Studies on the effect of foliar spray of Zn, Cu and B on growth, yield and fruit quality of sweet Orange (Citrus sinensis L.) cv. Mosambi. International Journal of Chemical Studies 2018; 6(5): 3260–3264.

38. Ilyas A, Ashraf MY, Hussain M, et al. Effect of micronutrients (Zn, Cu and B) on photosynthetic and fruit yield attributes of Citrus reticulata Blanco var. Kinnow. Pakistan Journal of Botany 2015; 47(4): 1241–1247.

39. Papadakis IE, Protopapadakis E, Therios IN, et al. Foliar treatment of Mn deficient ‘Washington navel’ orange trees with two Mn sources. Scientia Horticulturae 2005; 106(1): 70–75. doi: 10.1016/j.scienta.2005.02.015

40. Pestana M, Correia PJ, de Varennes A, et al. Effectiveness of different foliar iron applications to control iron chlorosis in orange trees grown on a calcareous soil. Journal of Plant Nutrition 2001; 24(4–5): 613–622. doi: 10.1081/pln-100103656

41. Hasani M, Zamani Z, Savaghebi G, et al. Effects of zinc and manganese as foliar spray on pomegranate yield, fruit quality and leaf minerals. Journal of Soil Science and Plant Nutrition 2012. doi: 10.4067/s0718-95162012005000009

42. Srivastava AK, Singh S. Citrus decline: Soil fertility and plant nutrition. Journal of Plant Nutrition 2009; 32(2): 197–245. doi: 10.1080/01904160802592706

43. Dutta P, Banik AK. Effect of foliar feeding of nutrients and plant growth regulators on physico-chemical quality of sardar guava grown in red and lateritic tract of west Bengal. Acta Horticulturae 2007; 735: 407–411. doi: 10.17660/actahortic.2007.735.57

44. Razzaq K, Khan AS, Malik AU, et al. Foliar application of zinc influences the leaf mineral status, vegetative and reproductive growth, yield and fruit quality of ‘kinnow’ mandarin. Journal of Plant Nutrition 2013; 36(10): 1479–1495. doi: 10.1080/01904167.2013.785567

45. Kaur N, Monga PK, Arora PK, et al. Effect of micronutrients on leaf composition, fruit quality and yield of kinnow mandarin. Journal of Applied and Natural Science 2015; 7(2): 639–643. doi: 10.31018/jans.v7i2.658

46. Singh J, Singh NP, Sangwan AK, et al. Standardization of litchi leaf nutrient composition for tissue analysis in sub tropics of India. Communications in Soil Science and Plant Analysis 2019; 50(22): 2889–2898. doi: 10.1080/00103624.2019.1689253