In higher eukaryotes, the genes’ architecture has become an essential determinant of the variation in the number of transcripts (expression level) and the specificity of gene expression in plant tissue under stress conditions. The modern rise in genome-wide analysis accounts for summarizing the essential factors through the translocation of gene networks in a regulatory manner. Stress tolerance genes are in two groups: structural genes, which code for proteins and enzymes that directly protect cells from stress (such as genes for transporters, osmo-protectants, detoxifying enzymes, etc.), and the genes expressed in regulation and signal transduction (such as transcriptional factors (TFs) and protein kinases). The genetic regulation and protein activity arising from plants’ interaction with minerals and abiotic and biotic stresses utilize high-efficiency molecular profiling. Collecting gene expression data concerning gene regulation in plants towards focus predicts an acceptable model for efficient genomic tools. Thus, this review brings insights into modifying the expression study, providing a valuable source for assisting the involvement of genes in plant growth and metabolism-generating gene databases. The manuscript significantly contributes to understanding gene expression and regulation in plants, particularly under stress conditions. Its insights into stress tolerance mechanisms have substantial implications for crop improvement, making it highly relevant and valuable to the field.

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