Biodegradable bacterial polyester, poly (hydroxybutyrate-co-hydroxyvalerate) copolymer, produced by moderately halophile bacterium Halomonas sp. PR-1 isolated from marine environment

Hui-Won Kim, Chol-Hyok Ri, Un-Ryong Han, Bong-Nan Kim, Chol-Min Pak, Se-Gol Kim, Gyu-Nam Ri

Article ID: 4399
Vol 7, Issue 1, 2024

VIEWS - 74 (Abstract) 42 (PDF)

Abstract


PHA (polyhydroxyalkanoate) production by halophiles has attracted much attention in recent years. It was certified that the halophile bacteria Halomonas sp. PR-1 isolated from saltern soil synthesised poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV) intracellularly from simple carbon substrate by means of FT-IR spectra analysis. The carbon and nitrogen source suitable for PHA production were selected as glucose and NH4Cl, respectively. The optimal ratio of glucose to NH4Cl was 20, when PHA content was 2.85 g/L and PHA yield was 50.85%. The optimal NaCl concentration for PHA biosynthesis was 30 g/L, when PHA yield was 63.3%. The halophile bacterium Halomonas sp. PR-1 was considered as a promising candidate for PHA production.


Keywords


bacterial polyester; poly (hydroxybutyrate-co-hydroxyvalerate); halophile; C/N ratio

Full Text:

PDF


References


1. Kushwah BS, Kushwah AVS, Singh V. RETRACTED ARTICLE: Towards understanding polyhydroxyalkanoates and their use. Journal of Polymer Research. 2016; 23(8). doi: 10.1007/s10965-016-0988-3

2. Mozejko-Ciesielska J, Kiewisz R. Bacterial polyhydroxyalkanoates: Still fabulous? Microbiological Research. 2016; 192: 271-282. doi: 10.1016/j.micres.2016.07.010

3. Raza ZA, Khalil S, Abid S. Recent progress in development and chemical modification of poly(hydroxybutyrate)-based blends for potential medical applications. International Journal of Biological Macromolecules. 2020; 160: 77-100. doi: 10.1016/j.ijbiomac.2020.05.114

4. Ray S, Kalia VC. Biomedical Applications of Polyhydroxyalkanoates. Indian Journal of Microbiology. 2017; 57(3): 261-269. doi: 10.1007/s12088-017-0651-7

5. Gadgil BST, Killi N, Rathna GVN. Polyhydroxyalkanoates as biomaterials. MedChemComm. 2017; 8(9): 1774-1787. doi: 10.1039/c7md00252a

6. Diniz MSF, Mourão MM, Xavier LP, et al. Recent Biotechnological Applications of Polyhydroxyalkanoates (PHA) in the Biomedical Sector—A Review. Polymers. 2023; 15(22): 4405. doi: 10.3390/polym15224405

7. Vanapalli KR, Sharma HB, Ranjan VP, et al. Challenges and strategies for effective plastic waste management during and post COVID-19 pandemic. Science of The Total Environment. 2021; 750: 141514. doi: 10.1016/j.scitotenv.2020.141514

8. Haque MdS, Sharif S, Masnoon A, et al. SARS-CoV-2 pandemic-induced PPE and single-use plastic waste generation scenario. Waste Management & Research: The Journal for a Sustainable Circular Economy. 2021; 39(1_suppl): 3-17. doi: 10.1177/0734242x20980828

9. Vicente D, Proença DN, Morais PV. The Role of Bacterial Polyhydroalkanoate (PHA) in a Sustainable Future: A Review on the Biological Diversity. International Journal of Environmental Research and Public Health. 2023; 20(4): 2959. doi: 10.3390/ijerph20042959

10. Meereboer KW, Misra M, Mohanty AK. Review of recent advances in the biodegradability of polyhydroxyalkanoate (PHA) bioplastics and their composites. Green Chemistry. 2020; 22(17): 5519-5558. doi: 10.1039/d0gc01647k

11. Kunioka M, Doi Y. Thermal degradation of microbial copolyesters: poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Macromolecules. 1990; 23(7): 1933-1936. doi: 10.1021/ma00209a009

12. Renard E, Walls M, Guérin P, et al. Hydrolytic degradation of blends of polyhydroxyalkanoates and functionalized polyhydroxyalkanoates. Polymer Degradation and Stability. 2004; 85(2): 779-787. doi: 10.1016/j.polymdegradstab.2003.11.019

13. Anjum A, Zuber M, Zia KM, et al. Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements. International Journal of Biological Macromolecules. 2016; 89: 161-174. doi: 10.1016/j.ijbiomac.2016.04.069

14. Thiré RMDSM, Arruda LC, Barreto LS. Morphology and thermal properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/attapulgite nanocomposites. Materials Research. 2011; 14(3): 340-344. doi: 10.1590/s1516-14392011005000046

15. Chen GQ, Jiang XR. Next generation industrial biotechnology based on extremophilic bacteria. Current Opinion in Biotechnology. 2018; 50: 94-100. doi: 10.1016/j.copbio.2017.11.016

16. Mitra R, Xu T, Xiang H, et al. Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory. Microbial Cell Factories. 2020; 19(1). doi: 10.1186/s12934-020-01342-z

17. Yin J, Chen JC, Wu Q, et al. Halophiles, coming stars for industrial biotechnology. Biotechnology Advances. 2015; 33(7): 1433-1442. doi: 10.1016/j.biotechadv.2014.10.008

18. Quillaguamán J, Guzmán H, Van-Thuoc D, et al. Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects. Applied Microbiology and Biotechnology. 2009; 85(6): 1687-1696. doi: 10.1007/s00253-009-2397-6

19. Sedlacek P, Slaninova E, Koller M, et al. PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances. New Biotechnology. 2019; 49: 129-136. doi: 10.1016/j.nbt.2018.10.005

20. Chen X, Yin J, Ye J, et al. Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Bioresource Technology. 2017; 244: 534-541. doi: 10.1016/j.biortech.2017.07.149

21. Tan D, Xue YS, Aibaidula G, et al. Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01. Bioresource Technology. 2011; 102(17): 8130-8136. doi: 10.1016/j.biortech.2011.05.068

22. Chen CW, Don TM, Yen HF. Enzymatic extruded starch as a carbon source for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei. Process Biochemistry. 2006; 41(11): 2289-2296. doi: 10.1016/j.procbio.2006.05.026

23. Kulkarni SO, Kanekar PP, Nilegaonkar SS, et al. Production and characterization of a biodegradable poly (hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake, India. Bioresource Technology 2010; 101: 9765-9771.

24. Kawata Y, Shi LH, Kawasaki K, et al. Taxonomic characterization and metabolic analysis of the Halomonas sp. KM-1, a highly bioplastic poly(3-hydroxybutyrate)-producing bacterium. Journal of Bioscience and Bioengineering. 2012; 113(4): 456-460. doi: 10.1016/j.jbiosc.2011.11.018

25. Gao S, Zhang LH. The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta Advanced Materials Research. 2014; 1033-1034: 306-310. doi: 10.4028/www.scientific.net/amr.1033-1034.306

26. Wei YH, Chen WC, Huang CK, et al. Screening and Evaluation of Polyhydroxybutyrate-Producing Strains from Indigenous Isolate Cupriavidus taiwanensis Strains. International Journal of Molecular Sciences. 2011; 12(1): 252-265. doi: 10.3390/ijms12010252

27. García-Torreiro M, Lu-Chau TA, Lema JM. Effect of nitrogen and/or oxygen concentration on poly(3-hydroxybutyrate) accumulation by Halomonas boliviensis. Bioprocess and Biosystems Engineering. 2016; 39(9): 1365-1374. doi: 10.1007/s00449-016-1612-y

28. Wei YH, Chen WC, Wu HS, et al. Biodegradable and Biocompatible Biomaterial, Polyhydroxybutyrate, produced by an Indigenous Vibrio sp. BM-1 Isolated from Marine Environment. Marine Drugs. 2011; 9(4): 615-624. doi: 10.3390/md9040615




DOI: https://doi.org/10.24294/nrcr.v7i1.4399

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Hui-Won Kim, Chol-Hyok Ri, Un-Ryong Han, Bong-Nan Kim, Chol-Min Pak, Se-Gol Kim, Gyu-Nam Ri

License URL: https://creativecommons.org/licenses/by/4.0/

This site is licensed under a Creative Commons Attribution 4.0 International License.