Soil erosion is characterized by the wearing away or loss of the uppermost layer of soil, driven by water, wind, and human activities. This process constitutes a significant environmental issue, with adverse effects on water quality, soil health, and the overall stability of ecosystems across the globe. This study focuses on the Anuppur district of Madhya Pradesh, India, employing the Revised Universal Soil Loss Equation (RUSLE) integrated with Geographic Information System (GIS) tools to estimate and spatially analyze soil erosion and fertility risk. The various factors of the model, like rainfall erosivity (R), soil erodibility (K), slope length and steepness (LS), conservation practices (P), and cover management factor (C), have been computed to measure annual soil loss in the district. Each factor was derived using geospatial datasets, including rainfall records, soil characteristics, a Digital Elevation Model (DEM), land use/land cover (LULC) data, and information on conservation practices. GIS methods are used to map the geographical variation of soil erosion, providing important information on the area’s most susceptible to erosion. The outcome of the study reveals that 3371.23 km², which constitutes 91% of the district’s total area, is identified as having mild soil erosion; in contrast, 154 km², or 4%, is classified as moderate soil erosion, while 92 km², representing 2.5%, falls under the high soil erosion category. Additionally, 50 km², or 1.35%, is categorized as very high soil erosion and around 30 km² of the study area is classified as experiencing severe soil erosion. The analysis further discovers that the annual soil loss in the district varies between 0 and 151 tons per hectare per year. This study indicates that most of the district is classified under low soil erosion; only a tiny fraction of the area is categorized as experiencing high and very high soil erosion. The study provides significant insights into soil erosion for policymakers and human society to bring their attention to the need for sustainable soil conservation practices in the undulating terrain/topography and agriculturally dominated district of Anuppur.