Infrared spectroscopy it is a useful technique, applied more and more, in the field of analytical research, in order to obtain the most useful information, from relatively easy-to-obtain samples for the characterization of a complex matrix such as liquid or biological tissue. The applications of vibrational spectroscopy are ever expanding, due to its non-destructive and versatile nature. Historically speaking, IR applications started with the pioneer works of Coblentz, in 1913. Since then, its development has been undeniably evidenced by an enormous number of review and research papers published every year, especially in biomedical analysis. Only in the last two years, 2021-2022, were published more than 20,000 papers.

This Special Issue aims to present, using reviews and original research, the current state-of-the-art in the field and some new applications of infrared spectroscopy in analysis in biomedical field.