Abstract Botrytis cinerea is a major disease in crop production. Chrysanthemum gray mold is easy to occur during chrysanthemum growing season. It can cause a lot of defoliation when serious, affecting plant flowering and reducing ornamental value. In this study, Arabidopsis thaliana ESB1 gene introduced into Agrobacterium tumefaciens was introduced into Agrobacterium tumefaciens and its identification after induction, and the sensitivity of chrysanthemum to antibiotics. It is expected that Agrobacterium tumefaciens will be transformed into chrysanthemum, result in transgenic chrysanthemum new lines resistance to disease, high quality, and high yield to lay the technical foundation. In this study, Arabidopsis thaliana ESB1 gene was introduced into Agrobacterium tumefaciens by freeze-thawing method. The PCR reaction was used to determine whether the ESB1 gene was successfully transferred into Agrobacterium tumefaciens and the susceptibility test of chrysanthemum to hygromycin was carried out to determine the critical tolerance concentration, Establishment of Chrysanthemum Transformation Receptor System. The result of PCR showed that the ESB1 gene was successfully transferred into Agrobacterium tumefaciens. The critical concentration of chrysanthemum to hygromycin was 30mg / L. rapid diffusion ability.