Silver nanoparticles functionalized in situ with D-limonene: Effect on antibacterial activity

Julián Echeverry-Chica, Andrea Naranjo-Díaz, Pedronel Araque-Marín

Article ID: 1685
Vol 5, Issue 2, 2022

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Abstract


This study focused on the formulation and characterization of silver nanoparticles (AgNP) functionalized with d-limonene. The nanoparticles were functionalized by phase inversion and the synthesis of the nanoparticles was performed in situ; particle size was determined by laser diffraction, zeta potential and optical colloidal stability using Multiscan 20 for a period of 24 hours at 37 °C; the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the formulated material on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Klebsiella oxytoca ATCC 700324, Enterococcus casseliflavus ATCC 700327, Escherichia coli BLEE, carbapenem-resistant Pseudomona aeruginosa were determined. The nanoparticles showed colloidal stability at a d-limonene concentration of 3.93%, silver ions at 1.61 × 10−3%, non-ionic adjuvant at 24% and ascorbic acid at 5.88%; citric acid/citrate (1:1) 0.48M for a pH of 4.5 was used as a buffer system. The formulation was classified as a polydisperse system (PD = 0.0851), with a zeta potential of −11.6 mV and average particle size of 81.5 ± 0.9 nm. A particle migration velocity of −0.199 ± 0.006 mm∙h−1, a constant transmission profile and backscattering profile with variations of 10% were evidenced, which represents a stable formulation. The nanoparticles presented an MIC and an MBC of 28 μg∙mL−1 (5.6 × 10−2% d-limonene and 4.7 × 10−5% AgNP) against all tested bacteria.


Keywords


Silver Nanoparticles; Phase Inversion; Bacterial Resistance; Minimum Inhibitory Concentration

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References


1. World Health Organization. Prioritization of pathogens to guide discovery, research and development of new antibiotics for drug-resistant bacterial infections, including tuberculosis. Switzerland: World Health Organization; 2017.

2. Ansari MA, Khan HM, Khan AA, et al. Gum arabic capped‐silver nanoparticles inhibit biofilm formation by multi‐drug resistant strains of Pseudomonas aeruginosa. Journal of Basic Microbiology 2014; 54(7): 688–699.

3. Rai MK, Deshmukh SD, Ingle AP, et al. Silver nanoparticles: The powerful nanoweapon against multidrug‐resistant bacteria. Journal of Applied Microbiology 2012; 112(5): 841–852.

4. Simões D, Miguel SP, Ribeiro MP, et al. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics 2018; 127: 130–141.

5. Pérez ZC, Torres CA, Nuñez MB. Antimicrobial activity and chemical composition of essential oils from Verbenaceae species growing in South America. Molecules 2018; 23(3): 544.

6. World Health Organization. Antibacterial agents in clinical development: an analysis of the antibacterial clinical development pipeline, including tuberculosis. Switzerland: World Health Organization; 2017.

7. Katz L, Baltz RH. Natural product discovery: Past, present, and future. Journal of Industrial Microbiology and Biotechnology 2016; 43(2–3): 155–176.

8. Sheikholeslami S, Mousavi SE, Ahmadi AH, et al. Antibacterial activity of silver nanoparticles and their combination with zataria multiflora essential oil and methanol extract. Jundishapur Journal of Microbiology 2016; 9(10): e36070.

9. Kaviya S, Santhanalakshmi J, Viswanathan B, et al. Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2011; 79(3): 594–598.

10. Ministry of Health. National plan of response to antimicrobial resistance. Strategic plan of the directorate of medicines and health technologies. Colombia: Ministry of Health; 2018.

11. World Health Organization. Global antimicrobial resistance surveillance system. Manual for the first phase of implementation. Switzerland: World Health Organization; 2017.

12. Morejón García M. Extended-spectrum betalactamases (in Spanish). Revista Cubana de Medicina 2013; 52(4): 272–280.

13. Suárez CJ, Kattán JN, Guzmán A, et al. Mechanisms of resistance to carbapenems in P. aeruginosa, Acinetobacter and Enterobacteriaceae and strategies for their prevention and control (in Spanish). Infectio 2006; 10(2): 85–93.

14. Torrenegra M, Pájaro N, Méndez L. In vitro antibacterial activity of essential oils from different species of the genus Citrus (in Spanish). Revista Colombiana de Ciencias Químico-Farmacéuticas 2017; 46(2): 160–175.

15. Shao P, Zhang H, Niu B, et al. Antibacterial activities of R-(+)-Limonene emulsion stabilized by Ulva fasciata polysaccharide for fruit preservation. International Journal of Biological Macromolecules 2018; 111: 1273–1280.

16. Mitropoulou G, Fitsiou E, Spyridopoulou K, et al. Citrus medica essential oil exhibits significant antimicrobial and antiproliferative activity. LWT 2017; 84: 344–352.

17. Pekmezovic M, Aleksic I, Barac A, et al. Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. FEMS Pathogens and Disease 2016; 74(8): ftw102.

18. Montironi ID, Cariddi LN, Reinoso EB. Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis. Revista Argentina de microbiologia 2016; 48(3): 210–216.

19. Chen G, Lin Y, Lin C, et al. Antibacterial activity of emulsified pomelo (Citrus grandis Osbeck) peel oil and water-soluble chitosan on Staphylococcus aureus and Escherichia coli. Molecules 2018; 23(4): 840.

20. Lou Z, Chen J, Yu F, et al. The antioxidant, antibacterial, antibiofilm activity of essential oil from Citrus medica L. var. sarcodactylis and its nanoemulsion. LWT 2017; 80: 371–377.

21. Al-Aamri MS, Al-Abousi NM, Al-Jabri SS, et al. Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman. Journal of Taibah University Medical Sciences 2018; 13(2): 108–112.

22. Rudakiya DM, Pawar K. Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: In vitro and in silico approaches. 3 Biotech 2017; 7(2): 1–12.

23. Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, et al. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. Journal of Saudi Chemical Society 2014; 18(4): 356–363.

24. McQuillan JS, Groenaga IH, Stokes E, et al. Silver nanoparticle enhanced silver ion stress response in Escherichia coli K12. Nanotoxicology 2012; 6(8): 857–866.

25. Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine: Nanotechnology, Biology and Medicine 2012; 8(1): 37–45.

26. Vilas V, Philip D, Mathew J. Essential oil mediated synthesis of silver nanocrystals for environmental, anti-microbial and antioxidant applications. Materials Science and Engineering: C 2016; 61: 429–436.

27. Zhang Z, Vriesekoop F, Yuan Q, et al. Effects of nisin on the antimicrobial activity of d-limonene and its nanoemulsion. Food chemistry 2014; 150: 307–312.

28. Ramirez LS, Castaño DM. Methodologies to evaluate in vitro antibacterial activity of plant-derived compounds (in Spanish). Scientia et Technica 2009; 15(42): 263–268.

29. Herrera ML. Antimicrobial sensitivity testing Laboratory methodology (in Spanish). Revista Médica del Hospital Nacional de Niños Dr. Carlos Sáenz Herrera 1999; 34: 33–41.

30. Jiménez N, Cienfuegos A, González G, et al. Culture media, identification tests and susceptibility tests (in Spanish). Medellín: Universidad de Antioquia; 2015.

31. Picazo JJ. Procedures in clinical microbiology (in Spanish). Recomendaciones de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Métodos básicos para el estudio de sensibilidad a los antimicrobianos. España: Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica; 2000.

32. CLSI M07. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 11th ed. Clinical and Laboratory Standards Institute; 2018.

33. CLSI M100. Performance standards for antimicrobial susceptibility testing. 28th ed. United States: Clinical and Laboratory Standards Institute; 2018.

34. Scandorieiro S, De Camargo L, Lancheros C, et al. Synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains. Frontiers in Microbiology 2016; 7: 760.

35. Rai M, Paralikar P, Jogee P, et al. Synergistic antimicrobial potential of essential oils in combination with nanoparticles: Emerging trends and future perspectives. International Journal of Pharmaceutics 2017; 519(1–2): 67–78.

36. Taghizadeh M, Solgi M. The application of essential oils and silver nanoparticles for sterilization of bermudagrass explants in in vitro culture. International Journal of Horticultural Science and Technology 2014; 1(2): 131–140.

37. Khalaf H, Sharoba A, El-Tanahi H, et al. Stability of antimicrobial activity of pullulan edible films incorporated with nanoparticles and essential oils and their impact on turkey deli meat quality. Journal of Food and Dairy Sciences 2013; 4(11): 557–573.

38. Bansod SD, Bawaskar MS, Gade AK, et al. Development of shampoo, soap and ointment formulated by green synthesised silver nanoparticles functionalised with antimicrobial plants oils in veterinary dermatology: Treatment and prevention strategies. IET Nanobiotechnology 2015; 9(4): 165–171.

39. Cui H, Zhang X, Zhou H, et al. Antimicrobial activity and mechanisms of Salvia sclarea essential oil. Botanical Studies 2015; 56(1): 1–8.

40. Huang D, Xu J, Liu J X, et al. Chemical constituents, antibacterial activity and mechanism of action of the essential oil from Cinnamomum cassia bark against four food-related bacteria. Microbiology 2014; 83(4): 357–365.

41. Li C, Yu J. Chemical composition: Antimicrobial activity and mechanism of action of essential oil from the leaves of Macleaya Cordata (Willd.) R. Br. Journal of Food Safety 2015; 35(2): 227–236.

42. Lakehal S, Meliani A, Benmimoune S, et al. Essential oil composition and antimicrobial activity of Artemisia herba-alba Asso grown in Algeria. Journal of Medicinal Chemistry 2016; 6(6): 435–439.

43. Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, et al. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. Journal of Saudi Chemical Society 2014; 18(4): 356–363.

44. Rhim J, Wang L, Hong S. Preparation and characterization of agar/silver nanoparticles composite films with antimicrobial activity. Food Hydrocolloids 2013; 33(2): 327–335.




DOI: https://doi.org/10.24294/can.v5i2.1685

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