Effect of an oscillating magnetic field in polymeric columns with magnetic nanoparticles

Violeta Maricela Dalgo Flores, Gabriela Cristina Chango Lescano, John Germán Vera Luzuriaga

Article ID: 1684
Vol 5, Issue 2, 2022

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Magnetite magnetic nanoparticles (MNP) exhibit superparamagnetic behavior, which gives them important properties such as low coercive field, easy superficial modification and acceptable magnetization levels. This makes them useful in separation techniques. However, few studies have experimented with the interactions of MNP with magnetic fields. Therefore, the aim of this research was to study the influence of an oscillating magnetic field (OMF) on polymeric monolithic columns with vinylated magnetic nanoparticles (VMNP) for capillary liquid chromatography (cLC). For this purpose, MNP were synthesized by coprecipitation of iron salts. The preparation of polymeric monolithic columns was performed by copolymerization and aggregation of VMNP. Taking advantage of the magnetic properties of MNP, the influence of parameters such as resonance frequency, intensity and exposure time of a OMF applied to the synthesized columns was studied. As a result, a better separation of a sample according to the measured parameters was obtained, so that a column resolution (Rs) of 1.35 was achieved. The morphological properties of the columns were evaluated by scanning electron microscopy (SEM). The results of the chromatographic properties revealed that the best separation of the alkylbenzenes sample occurs under conditions of 5.5 kHz and 10 min of exposure in the OMF. This study constitutes a first application in chromatographic separation techniques for future research in nanotechnology.


Capillary Liquid Chromatography; Oscillation Frequency; Nanoparticles; Superparamagnetic

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1. Lynch KB, Ren J, Beckner MA, et al. Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications. Analytica Chimica Acta 2019; 1046: 48–68.

2. Dores‐Sousa JL, Fernández‐Pumarega A, De Vos J, et al. Guidelines for tuning the macropore structure of monolithic columns for high‐performance liquid chromatography. Journal of Separation Science 2019; 42(2): 522–533.

3. Liu L, Yang C, Yan X. Methacrylate-bonded covalent-organic framework monolithic columns for high performance liquid chromatography. Journal of Chromatography A 2017; 1479: 137–144.

4. Wang R, Li W, Chen Z. Solid phase microextraction with poly (deep eutectic solvent) monolithic column online coupled to HPLC for determination of non-steroidal anti-inflammatory drugs. Analytica Chimica Acta 2018; 1018: 111–118.

5. Sharma G, Tara A, Sharma VD. Advances in monolithic silica columns for high-performance liquid chromatography. Journal of Analytical Science and Technology 2017; 8(1): 1–11.

6. Kartsova LA, Bessonova EA, Somova VD. Hydrophilic interaction chromatography. Journal of Analytical Chemistry 2019; 74(5): 415–424.

7. Buszewski B, Szumski M. Study of bed homogenity of methacrylate-based monolithic columns for micro-HPLC and CEC. Chromatographia 2004; 60(1): S261–S267.

8. Svec F, Lv Y. Advances and recent trends in the field of monolithic columns for chromatography. Analytical Chemistry 2015; 87(1): 250–273.

9. Poupart R, Grande D, Carbonnier B, et al. Porous polymers and metallic nanoparticles: A hybrid wedding as a robust method toward efficient supported catalytic systems. Progress in Polymer Science 2019; 96: 21–42.

10. Li Z, Rodriguez E, Azaria S, et al. Affinity monolith chromatography: A review of general principles and applications. Electrophoresis 2017; 38(22–23): 2837–2850.

11. Gama MR, Rocha FRP, Bottoli CBG. Monoliths: synthetic routes, functionalization and innovative analytical applications. TrAC Trends in Analytical Chemistry 2019; 115: 39–51.

12. Terborg L, Masini JC, Lin M, et al. Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode. Journal of Advanced Research 2015; 6(3): 441–448.

13. Aqel A. Using of nanomaterials to enhance the separation efficiency of monolithic columns. Nanomaterials in Chromatography. Elsevier; 2018. p. 299–322.

14. Soriano ML, Zougagh M, Valcárcel M, et al. Analytical nanoscience and nanotechnology: Where we are and where we are heading. Talanta 2018; 177: 104–121.

15. Carrasco-Correa EJ, Ramis-Ramos G, Herrero-Martínez JM. Hybrid methacrylate monolithic columns containing magnetic nanoparticles for capillary electrochromatography. Journal of Chromatography A 2015; 1385: 77–84.

16. Barbosa-Canovas G. Food Engineering-Volume III. Washington: EOLSS Publications; 2009.

17. Aguilera-Díaz JD, Parra-Pérez A. Design and construction of a magnetic field generator with intensity, direction and frequency control (in Spanish) [Undergraduate thesis]. Bogotá: Universidad Santo Tomas de Aquino; 2015.

18. Prieto A, Pereda JA, González O. Opencourseware electricity and magnetism [Internet]. University of Cantabria; 2010. Available from: https://ocw.unican.es/course/view.php?id=197.

19. Tipler PA, Mosca G. Electricity and magnetism. In: Física para la ciencia y la tecnología. Barcelona-Bogotá: Reverté; 2005. p. 878–897.

20. Saien J, Bamdadi H, Daliri S. Liquid-liquid extraction intensification with magnetite nanofluid single drops under oscillating magnetic field. Journal of Industrial and Engineering Chemistry 2015; 21: 1152–1159.

21. Jiménez IR, Gorbeña JCR, Félix ST. [Influence of variable sine wave magnetic field of (22–52) khz and 100 milligauss magnetic induction, on the growth of Lactobacillus plantarum used as a probiotic in food (in Spanish). Biotempo 2017; 14(1): 49–55.

22. Petro M, Svec F, Fréchet JMJ. Molded continuous poly (styrene-co-divinylbenzene) rod as a separation medium for the very fast separation of polymers Comparison of the chromatographic properties of the monolithic rod with columns packed with porous and non-porous beads in high-performance liquid chromatography of polystyrenes. Journal of Chromatography A 1996; 752(1–2): 59–66.

23. Yang C, Wang G, Lu Z, et al. Effect of ultrasonic treatment on dispersibility of Fe3O4 nanoparticles and synthesis of multi-core Fe3O4/SiO2 core/shell nanoparticles. Journal of Materials Chemistry 2005; 15(39): 4252–4257.

24. Yu Q, Dave RN, Zhu C, et al. Enhanced fluidization of nanoparticles in an oscillating magnetic field. AIChE Journal 2005; 51(7): 1971–1979.

25. Van Ommen JR, Valverde JM, Pfeffer R. Fluidization of nanopowders: A review. Journal of Nanoparticle Research 2012; 14(3): 1–29.

26. Pantoja JMM. Microwave engineering: experimental techniques (in Spanish). Pearson Educación; 2002.

27. Rios A, Zougagh M. Recent advances in magnetic nanomaterials for improving analytical processes. TrAC Trends in Analytical Chemistry 2016; 84: 72–83.

28. Roig C. Validation of a high resolution liquid chromatography method (HPLC) for the determination of ivabradine tablets. Mem. Instituto de Investig. en Ciencias de la Salud 2012: 63–70.

29. Bose A. HPLC calibration process parameters in terms of system suitability test. Austin Chromatogr 2014; 1(2): 1–4.

30. Jiles DC, Atherton DL. Theory of ferromagnetic hysteresis. Journal of Applied Physics 1984; 55(6): 2115–2120.

31. Dadoo R, Zare RN, Yan C, et al. Advances in capillary electrochromatography: Rapid and high-efficiency separations of PAHs. Analytical Chemistry 1998; 70(22): 4787–4792.

DOI: http://dx.doi.org/10.24294/can.v5i2.1684


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