Culture of C6 glioma cells and establishment of model

Shumin Liang, Yijun Chen, Kaifei Wang

Article ID: 307
Vol 1, Issue 1, 2018, Article identifier:

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Objectives: To establish a simple, reliable, stable rat C6 glioma model, and to provide an operating platform for


studying the pathogenesis and prevention and treatment of glioma. Methods: C6 rat glioma cells were cultured in vitro and cultured in the right caudate putamen of SD rats. After implantation, the survival status of rats was observed and the pathological sections were made. HE is dyeing under light microscope. Results: After 6 days of inoculation of C6 glioma cells, the survival status was good at about 7 days, and the intracranial hypertension was more obvious at about 14 days. Most of them were in the endangered state. In the 18 specimens of the rats, except for the accidental death, the other tumor formation rate was 75%. The tumor structure was obviously displaced with the prolongation of the age of the tumor. HE staining was used to observe the formation of glioma in rat brain. Conclusion: The animal model of C6 glioma is reliable and stable. Its tumor growth characteristics and pathological features are similar to those of human glioma, which can be used as an ideal model for clinical study of glioma.

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Ji X, et al. Observation on the growth of intracranial inoculation of stereotactic rat gliomas. Journal of Neurology and Neurology, 2005,12 (5): 311-314.

Wu J, et al. Establishment of rat C6 glioma model and observation of intracranial tumor growth [J]. Journal of the Fourth Military Medical University, 2000,21 (3): 307-310.

Fang S, et al. Study on apoptosis of C6 glioma cells induced by local thermochemotherapy [J]. Chinese Journal of Clinical Neurosurgery, 2003, 8 (2): 127-130.

Zhu H, et al. Study on animal model of glioma and its application [J]. Zoological Research 2012, Jun.33 (3): 337-342.

Qiu D, et al. Effects of indwelling time after injection on C6 rat glioma model [J]. Journal of Chongqing Medical University, 2008,33 (10): 1260- 1263.

Bai Y, et al. Establishment of rat brain glioma animal model and its morphological observation [J]. Journal of Xuzhou Medical College, 2004,24 (4): 331- 334.

Watanabe K, et al. Feasibility and limitations of the rat model by C6 gliomas implanted at the subcutaneous region [J]. Neurol Res, 2002, 24: 485-490.

Del Duca D, et al. Spheroid preparation from hanging drops: characterization of a model of brain tumor invasion [J]. J

Neurooncol, 2004, 67: 295-330.

Kondziolka D, et al. Radiobiology of radiosurgerg: Part. The rat C6 glioma model [J]. Neurosurgery, 2007, 31 (2): 280- 287.

Kinoshita N, et al. Usefulness of basic fibroblast growth factor for radiation-exposed tissue [J]. Wound Repair Regen, 2012,20 (1): 91-102.



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