Cloning of Mouse β-actin Gene

Chengjun Ji, Mingli Wang, Xuedong Wen

Article ID: 298
Vol 1, Issue 1, 2018

VIEWS - 1375 (Abstract) 421 (PDF)

Abstract


β-actin gene is a kind of actin, in a variety of cells and tissues, β-actin gene expression is relatively stable [1], and β-actin gene in nuclear DNA has multiple copies, increased The detection rate of nuclear DNA [2], which is often used as an internal reference in PCR. In this experiment, the β-actin gene with good purity was obtained by PT-PCR, amplification, cloning, screening and PCR identification and restriction enzyme digestion of the RNA extracted from the mouse liver, which laid the foundation for the later experiment.

 


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References


1. The specific heat of the PCR reaction system of mouse housekeeping gene β-actin was determined by DSC. The Institute of Food and Cryogenic Biotechnology, Shanghai University of Science and Technology, Food Science - Bioengineering 348, Section 10 Period, 2007, vol.28.

2. Yuan Zhengjie, Zhang Gengsheng, Sun Rui, et al., the internal reference method in the mitochondrial DNA purity identification of the application, Journal of wheat crop, 2009,29 (6): 1088-1093.

3. Yang Aifu, Zhou Zunchun, Dong Ying, et al. Comparative analysis of the expression stability of cytb and β-actin genes in the genus Eriocheir sinensis, China Agricultural Science and Technology Herald, 2010.12 (1); 79-84.

4. http: //www.baidu.com Baidu Encyclopedia

5. http://jpkc.gdpu.cn/2jpfzswx/Course/Content.asp?c=345u0026a=587u0026todo=show plasmid DNA extraction and purification of alkaline lysis of Guangdong Institute of Molecular Biology Course website.




DOI: https://doi.org/10.24294/as.v1i1.298

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