Stem transcriptome of cold stressed Eucalyptus globulus and E. urograndis

Pedro Araújo

Abstract


Eucalyptus is an important source of cellulose and a widely cultivated plant. Biotechnology tools can save time spent in breeding and transcriptomic approaches generate a gene profile that allows the identification of candidates involved in processes of interest. RNA-seq is a commonly used technology for transcript analysis and it provides an overview of regulatory pathways. Here, we selected two contrasting Eucalyptus species for cold acclimatization and focused in responsive genes under cold condition aiming woody properties – lignin and cellulose. The number of differentially expressed genes identified in stem sections were 3.300 in Eucalyptus globulus and 1370 in Eucalyptus urograndis. We listed genes with expression higher than 10 times including NAC, MYB and DUF family members. The GO analysis indicates increased oxidative process for E. urograndis. This data can provide information for more detailed analyses for breeding, especially in perennial plants.


Keywords


Eucalyptus; RNA-seq; cold stress; cell wall

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References


González-Garcíaa S, Moreira MT, Feijoo G. Environmental aspects of eucalyptus based ethanol production and use. Science of The Total Environment 2012; 438:1-8.

Pleguezuelo CRR, Zuazo VHD, Bielders C, et al. Bioenergy farming using woody crops. A review. Agronomy for Sustainable Development 2015; 35(1):95-119.

Davidson N, Reid JB. Frost as a factor influencing the growth and distribution of subalpine eucalypts. Australian Journal Botany 1985; 33:657-667.

Moura JC, Bonine CA, Viana JOF, et al. Abiotic and biotic stresses and changes in the lignin content and composition in plants. Journal Integrative Plant Biology 2010; 52(4):360-376.

Eldridge K, Davidson J, Hardwood C, et al. Eucalypt Domestication and Breeding. Oxford Science Publications 1993; USA: Clarendon Press.

Carbonnier L, Marques C, Coutinho J, et al. The future of Eucalyptus plantations. In Borralho N, Pereira J, Marques C, et al. IUFRO on silviculture and improvement of Eucalypts: Eucalyptus in a changing world. Raiz Instituto 2004; 29.

Almeida AC, Siggins A, Batista TR, et al. Mapping the effect of spatial and temporal variation in climate and soils on Eucalyptus plantation production with 3-PG, a process-based growth model. Forest Ecology and Management 2010; 259(9):1730-1740.

Chinnusamy V, Zhu J, Zhu JK. Cold stress regulation of gene expression in plants. Trends in Plant Science 2007; 12(10):444-451.

Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nature reviews Genetics 2009; 10(1):57-63.

Zhan X, Zhu JK, Lang Z. Increasing Freezing Tolerance: Kinase Regulation of ICE1. Developmental Cell 2015; 32:257-258.

Shi Y, Huang J, Sun T, et al. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana. Journal Integrative Plant Biology 2016; 59(2):118-133.

Tibbits WN, White TL, Gary RH, et al. Genetic variation in frost resistance of Eucalyptus

globulus ssp. globulus assessed by artificial freezing in winter. Australian Journal of Botany 2006; 54(6):521–529.

Boerjan W, Ralph J, Baucher M. Lignin biosynthesis. Annual Review of Plant Biology 2003; 54:519-546.

Zhong R, Lee C, Zhou J, et al. A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis. Plant Cell 2008; 20(10):2763-2782.

Agarwal M, Hao Y, Kapoor A, et al. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry 2006; 281(49):37636-37645.

Jin H, Cominelli E, Bailey P, et al. Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. Embo Journal 2000; 19(22):6150-6161.

McFarlane HE, Döring A, Persson S. The Cell Biology of Cellulose Synthesis. Annual Review of Plant Biology 2014; 65:69-94.

Nakano Y, Yamaguchi M, Endo H, et al. NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants. Frontiers in Plant Science 2015; 6(288):1-18.

Marjamaa K, Kukkola EM, Fagerstedt KV. The role of xylem class III peroxidases in lignification. Journal of Experimental Botany 2009; 60(2):367–376.

Sewelam N, Kazan K, Schenk PM. Global Plant Stress Signaling: Reactive Oxygen Species at the Cross-Road. Frontiers in Plant Science 2016;7:187.

Schuetz M, Smith R, Ellis B. Xylem tissue specification, patterning, and differentiation mechanisms. Journal of Experimental Botany 2013;64(1):11–31.

Kreps JA, Wu Y, Chang HS, et al. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiology 2002; 130(4):2129-2141.

Navarro M, Ayax C, Martinez Y, et al. Two EguCBF1 genes overexpressed in Eucalyptus display a different impact on stress tolerance and plant development. Plant Biotechnology Journal 2011; 9(1):50-63.

Akhtar M, Jaiswal A, Taj G, et al. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. Journal of Genetics 2012; 91(3):385-395.

Chinnusamy V, Ohta M, Kanrar S, et al. ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes & Development 2003; 17(8):1043-1054.

Baucher M, Chabbert B, Pilate G, et al. Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar. Plant Physiology 1996; 112:1479-1490.

García JR, Anderson N, Le-Feuvre R, et al. Rescue of syringyl lignin and sinapate ester biosynthesis in Arabidopsis thaliana by a coniferaldehyde 5-hydroxylase from Eucalyptus globulus. Plant Cell Reports 2014; 33(8):1263-1274.

Lee HW, Kim J. EXPANSINA17 up-regulated by LBD18/ASL20 promotes lateral root formation during the auxin response. Plant & Cell Physiology 2013; 54(10):1600-1611.

Chen S, Ehrhardt DW, Somerville CR. Mutations of cellulose synthase (CESA1) phosphorylation sites modulate anisotropic cell expansion and bidirectional mobility of cellulose synthase. Proceedings of the National Academy of Sciences of the United States of America 2010; 107(40):17188-17193.

Lan W, Lu F, Regner M, et al. Tricin, a Flavonoid Monomer in Monocot Lignification. Plant Physiology 2015; 167:1284-1295.




DOI: http://dx.doi.org/10.24294/th.v1i2.889

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