Dear Colleagues,

Biomolecule chromatography is critical in the purification, analysis, and characterization of various biomolecules such as proteins, nucleic acids, carbohydrates, and lipids. This abstract discusses the advancements, applications, and future prospects of biomolecule chromatography, emphasizing its importance in sectors such as medicines, biotechnology, proteomics, and biochemistry. Significant advances in chromatographic techniques have been made throughout the years, allowing for improved separation and purification of biomolecules. Size exclusion chromatography, ion exchange chromatography, affinity chromatography, hydrophobic interaction chromatography, and reversed-phase chromatography are examples of these techniques. Novel stationary phases, improved column designs, and high-resolution instruments have all led to improved chromatographic performance and separation efficiency.

Biomolecule chromatography has a wide range of applications. Chromatography is essential in the pharmaceutical sector for the isolation and purification of medicinal proteins, peptides, and antibodies. It ensures the elimination of contaminants and aggregates, resulting in biotherapeutics that are both safe and efficacious. Chromatography is used in biotechnology to separate and purify recombinant proteins and enzymes, which aids in downstream processing and bioproduction. In proteomics and metabolomics research, chromatographic techniques are widely utilised for protein identification, biomarker discovery, and metabolic profiling. The future of biomolecule chromatography seems bright. Ongoing research is centred on the development of innovative stationary phases, such as monolithic columns and mixed-mode chromatography that provide higher selectivity and sample throughput. Furthermore, combining chromatography with additional analytical techniques, such as mass spectrometry and high-throughput screening, allows for more complete biomolecular analysis.

Additionally, attempts are being made to improve the sustainability and cost-effectiveness of chromatographic operations by employing alternative solvents, green stationary phases, and continuous chromatography systems. Finally, biomolecule chromatography has completely transformed the field of biomolecular analysis and purification. Its broad range of applications in a variety of fields emphasizes its significance in promoting scientific research, pharmaceutical development, and biotechnological innovation. Biomolecule chromatography is likely to continue playing an important role in unraveling the intricacies of biomolecules and generating discoveries in life sciences and healthcare, thanks to ongoing advancements and promising future possibilities.