Validation and efficacy of various decontamination solutions in eliminating acephate residues from okra
Vol 7, Issue 2, 2024
VIEWS - 51 (Abstract) 30 (PDF)
Abstract
The experiments were carried out to validate an analytical method and to examine the impact of various decontaminating solutions on the removal of acephate residues from okra. Acephate analysis was performed using HPLC-UV, and sample extraction was done using the QuEChERS method. Method validation encompassed assessing specificity, linearity, precision, accuracy, as well as limits of detection (LOD) and quantification (LOQ). The method exhibited excellent linearity with R2 values ≥ 0.99. LOD and LOQ were determined at 0.5 µg mL−1 and 2 µg mL−1, respectively. The results indicated average recoveries ranging from 80.2% to 83.3% with a % RSD below 5%. The decontamination procedures include rinsing with running tap water, soaking in lukewarm water, 2% CH3COOH, 1% NaCl, 5% NaHCO3, 0.01% KMnO4, and in commercially available decontamination products such as nimwash, veggie clean, and arka herbiwash for a duration 10 minutes. Among all the treatments, soaking in nimwash solution showed remarkable effectiveness (96.75% removal), followed by veggie clean (94.97% removal) and arka herbiwash (95.80% removal). Washing okra samples in running tap water was found to be the least effective compared to other treatments.
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DOI: https://doi.org/10.24294/th.v7i2.4507
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