Researchers at the University of Zurich have developed a novel method for analyzing cells and their components called Iterative Indirect Immunofluorescence Imaging (4i). This innovation greatly refines the standard immunofluorescence imaging technique used in biomedicine and provides clinicians with an enormous amount of data from each individual sample. 4i makes it possible to observe the spatial distribution of at least 40 proteins and their modifications in the same cell for hundreds of thousands of cells simultaneously at various levels, from the tissue down to the organelle level.