Native and lyophilized shredded muscle tissue of beef and its main ingredients (muscle fibers, stroma, stroma proteins) prepared via consecutive treatment with water, saline (Weber solution ) and alkaline (0.6 M NaOH) solutions as well as colloidal solutions-extracts of sarcoplasmic substances, actomyosin and mucopolysaccharide complexes are comparatively studies by attenuated total internal reflection infrared (ATIR) and diffuse reflectance electronic spectroscopy. A high sensitivity, efficiency, extended options and advanced prospects of diffuse spectroscopy are considered in respect of the characterization of  the component composition of complex solid-state and liquid biological systems in medical and veterinary practice.

The complex of investigations of optical characteristics of controlled objects and spectral symmetric-wave and two-colour compensating thermometry (SWT and TCCT) influence on their errors of method and instrumental errors is performed. Advantages of these new methods in the field of errors of method, in comparison with known spectral and also classical energy and spectral ratio thermometry are proved. It is established that right use of the SWT and the TCCT, which takes into account thermometrical conditions, allows to completely exclude methodical and instrumental components from the errors of optical temperature measurements. 

Spectral thermometry is based on remote quantitative determination of the spectral emissivity distributions of controlled objects. Using these distributions and well-known relationships between the spectral coefficients of emission, absorption, reflection and transmission we can determine macro-optical characteristics of these objects and remotely investigate them.