Heavy metal-induced oxidative stress and DNA damage as shown by RAPD-PCR in leaves of Elodea canadensis

Dilek Demirezen Yilmaz, Nuri Ercan, Fahriye Sumer Ercan

Article ID: 724
Vol 3, Issue 1, 2020

VIEWS - 694 (Abstract) 273 (PDF)

Abstract


The objective of the study was to evaluate the antioxidant response and DNA damage of heavy metals (Cd, Cu and Cr) in Elodea canadensis. Superoxide dismutase (SOD), catalase (CAT), Glutathione reductase (GR) and lipid peroxidation levels of leaves of Elodea canadensis which was exposed to different concentrations of heavy metals (Cd: 2, 5, 10, 15, 25 ppb; Cu: 200, 500, 1000, 2500 ppb and Cr: 1, 3, 10, 15, 25 ppb) in a hydroponic culture were determined spectrophotometrically. The highest induction in SOD and CAT activities were determined at highest concentration of heavy metals. The Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) technique was used to investigate the variation of DNA banding patterns of samples that exposed to different concentrations of heavy metals. Changing in band intensity and the gain and loss of bands were demonstrated the genotoxic effect of heavy metals. Bioaccumulation, oxidative responses and DNA damages were shown that Elodea canadensis represents a useful bioindicator.


Keywords


Elodea Canadensis; Oxidative Stress; DNA Damage; RAPD-PCR

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References


1. Dixit V, Pandey V, Shyam R. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). Journal of Experimental Botany 2001; 52(358): 1101–1109.

2. Verma S, Dubey SR. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science 2003; 164(4): 645–655.

3. Jimi S, Uchiyama M, Takaki A, et al. Mechanism of cell death induced by cadmium and arsenic. Annals of the New York Academy of Sciences 2004; 1011: 325–331.

4. Mithofer A, Schulze B, Boland W. Biotic and heavy metal stress response in plants: Evidence for common signals. FEBS Letters 2004; 566: 1–5.

5. Ganesh KS, Baskaran L, Rajasekaran S, et al. Chromium stress induced alterations in biochemical and enzyme metabolism in aquatic and terrestrial plants. Colloids and Surfaces Biointerfaces 2008; 63: 159–163.

6. Steinkellner H, Kassie F, Knasmuiler S. Tradescaotia micronucleus assay for the assessment of the clastogenicity of Austian water. Mutation Research 1999; 426: 113–116.

7. Angelis JK, McGuffie M, Menke M, et al. Adaptation to alkylation damage in DNA measured by the comet assay. Environmental and Molecular Mutagenesis 2000; 36(2):146–150.

8. Labra M, Grassi F, Imazio S, et al. Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L. Chemosphere 2004; 54: 1049–1058.

9. Atienzar FA, Cordi B, Donkin MB, et al. Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Palmaria palmate. Aquatic. Toxicology 2000; 50(1-2): 1–12.

10. Savva D. DNA fingerprinting as a biomarker assay in ecotoxicology. Toxicology and Ecotoxicology News 1996; 3: 110–114.

11. Savva D. Use of DNA fingerprinting to detect genotoxic effects. Ecotoxicology and Environmental Safety 1998; 41(1): 103–106.

12. Shikazono N, Zakir HM, Sudo Y. Zinc contamination in river water and sediments at Taisyu Zn–Pb mine area, Tsushima Island, Japan. Journal of Geochemical Exploration 2008; 98(3): 80–88.

13. PavlíkováD, Pavlik M, StaszkováL, et al. Glutamate kinase as a potential biomarker of heavy metal stress in plants. Ecotoxicology and Environmental Safety 2008; 70(2): 223–230.

14. Khan NA. Abiotic Stress and Plant Responses. In: Singh S (editor). New Delhi: IK International; 2008.

15. Rogers SO, Bendich AJ. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 1985; 5: 69–76. Available from: https://doi.org/10.1007/BF00020088.

16. Parlak KU, Yılmaz DD. Response of antioxidant defences to Zn stress in three duckweed species. Ecotoxicology and Environmental Safety 2012; 85(1): 52–58.

17. Razinger J, Dermastia M, Koce JD, et al. Oxidative stress in duckweed (Lemna minor L.) caused by short-term cadmium exposure. Environmental Pollution 2008; 153(3): 687–694.

18. Hou W, Chen X, Song G, et al. Effect of copper and cadmium on heavy metal polluted water body restoration by duckweed (Lemna minor). Plant Physiology and Biochemistry 2007; 45(1): 62–69.

19. Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 1971; 44(1): 276–287.

20. Aebi H. Catalase in vitro. Methods in Enzymology 1984; 105: 121–176.

21. Enan MR. Application of random amplified polymorphic DNA (RAPD) to detect the genotoxic effect of heavy metals. Biotechnology and Applied Biochemistry 2006; 43(3): 147–154.

22. Liu W, Yang Y, Li P, et al. Risk assessment of cadmium contaminated soil on plant DNA damage using RAPD and physiological indices. Journal of Hazardous Materials 2009; 161: 878–883.

23. Conte C, Mutti I, Puglisi P, et al. DNA finger printing analysis by a PCR based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere 1998; 37: 2739–2749.

24. Liu W, Li P, Qi X, et al. DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis. Chemosphere 2005; 61:158–167.

25. Aust AE, Eveleigh JF. Mechanism of DNA oxidation. Proceedings of the Society for Experimental Biology and Medicine 1999; 222(3): 246–252.

26. Bišová K, Hendrychová J, Cepák V, et al. Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda. Folia Microbiol 2003; 48 (6): 805–816.

27. Atesi I, Suzen HS, Aydin A, Karakaya A. The oxidative DNA base damage in tests of rats after intraperitoneal cadmium injection. Biometals 2004; 17(4): 371–377.

28. Gupta DK, Nicoloso FT, Schetinger MRC, et al. Antioxidant defence mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. Journal of Hazardous Materials; 172(1): 479–484.

29. Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 2002; 7(9): 405–410.

30. Yılmaza DD, Parlak KU. Changes in proline accumulation and antioxidative enzyme activitiesin Groenlandia densa under cadmium stress. Ecological Indicators 2011; 11(2): 417–423.

31. Sharma P, Dubey RS. Involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum. Plant Cell Reports 2007; 26: 2027–2038. Available from: https://doi.org/10.1007/s00299-007-0416-6.

32. Shankar AK, Cervantes C, Loza-Tavera H, et al. Chromium toxicity in plants. Environment International 2005; 31(5): 739–753.

33. Willekens H, Chamnongpol S, Davey M, et al. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. The EMBO Journal 1997; 16(16): 4806–4816. doi: 10.1093/emboj/16.16.4806.




DOI: https://doi.org/10.24294/ace.v3i1.724

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