Applied Chemical Engineering (Transferred)

In oil industry microbiologically influenced corrosion plays a key role since it costs a lot of money yearly. This kind of corrosion is mainly induced by the microbial biofilms occurring on the metal surface and their metabolites that modify the electrochemical conditions from metal-solution interface. This study focused on the evaluation of the antimicrobial activity of essential oil of Lippiagracilis Schauer over Clostridium bifermentans isolated from ballast of ship transporter of crude oil as well as against fungi occurring on microbial biofilms. Additionally, it was evaluated the influence of the essential oil on the corrosion of AISI 1020 carbon steel by electrochemical and gravimetric techniques. A minimum inhibitory concentration of the 20.0 μg·L^-1 was obtained for the essential oil over the C. bifermentans that was the same used for investigating the biocide activity against fungal biofilms for different contact time. Results showed that colony former unit (CFU) for fungi reduced to zero after 120 minutes exposition to the essential oil. Also, the essential oil of L. gracilis Schauer showed a quite good potential to control effectively the growth of C. bifermentans. Electrochemical polarization and gravimetry assays showed that essential oil of L. gracilis Schauer at concentration of 60 µg·L^-1 was efficient to inhibit the corrosion of AISI 1020 carbon steel. L. gracilis Schauer essential oil acted as a powerful biocide.

1. Videla HA, Herrera LK. Understanding microbial inhibition of corrosion. A comprehensive overview. Internat. Biodet. & Biodegrad. 2009; 63: 896–900.

2. Gu J. New understandings of biocorrosion mechanisms and their classifications. J. Microb. Biochem. Technol. 2012; 4: 3–4.

3. Lieser MJ, Stek C. Composite and the Future of Society: preventing a legacy of costly corrosion with modern materials. Owens Corning. 2010; 1–17.

4. Magot M. Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek. 2000; 77: 103–116.

5. Jan-Roblero J, Romero J, Amaya JM, et al. Phylogenetic characterization of a corrosive consortium isolated from a sour gas pipeline. Appl. Microbial Biotechnology. 2008; 64: 862–867.

6. Khelifi E, Bouallagui H, Fardeau ML, et al. Fermentative and sulphate-reducing bacteria associated with treatment of an industrial dye effluent in an up-flow anaerobic fixed bed bioreactor. Bioch. Engin. J. 2009; 45: 136–144.

7. Duque Z, Ibars JR, Sarro MI, et al. Comparison of sulphite corrosivity of sulphate- and non-sulphate-reducing prokaryotes isolated of oilfield injection water. Material and Corrosion. 2011; 62: 6291–6298.

8. Barloy F, Delécluse A, Nicolas L, et al. Cloning and expression of the first anaerobic toxin gene from Clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to Bacillus thuringiensis delta-endotoxins. J. Bacter. 1996; 11: 3099–3105.

9. Larsen LM, Nielsen TH, Ploger A, et al. Rapid and efficient method for the isolation and characterization of plant aromatic choline enterases. J. Chromat. 1988; 450: 121–131.

10. Little BJ, Lee JS. Microbiologically Influenced Corrosion. Hoboken, New Jersey: John Wiley & Sons, Inc.; 2007. p. 22.

11. Lanneluc I, Langumier M, Sabot R, et al. On the bacterial communities associated with the corrosion product layer during the early stages of marine corrosion of carbon steel. J. Biodet. Biodeg. 2015; 99: 55–65.

12. Gu J. Theoretical modeling of the possibility of acid producing bacteria causing fast pitting biocorrosion. J. Microb. Biochem. Technol. 2014; 6: 2.

13. Mattos FJA, Machado MIL, Craveiro AA, et al. Medicinal plants Northeast Brazil containing thymol and carvacrol-Lippia sidoides Cham. and L. gracilis H.B.K (Verbeneacea). J. Essent. Oils Resear. 1999; 11: 666–668.

14. Marreto RN, Almeida EECV, Alves PB, et al. Thermal analysis and gas chromatography coupled mass spectrometry analyses of hydroxypropyl-β-cyclodextrin inclusion complex containing Lippia gracilis essential oil. Thermoc. Acta 2008; 475: 53–58.

15. Pimenta M, Fernandes LS, Pereira UJ, et al. Flowering, germination and rooting of cuttings of Lippia L. (Verbenaceae). Rev. Brasileira de Botânica 2007; 30: 211–220..

16. Oliveira OR, Terao D, Carvalho ACPP, et al. Effect of essential oil from genus Lippia plants over the control of fungi contaminants on the micro propagation of plants. Revista Ciência Agronômica 2008; 39: 94–100.

17. Van DLH, Kratz PH. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromat. A 1963; 11: 463.

18. GenBank [Internet]. Bethesda: National Library of Medicine; 2009 [accessed 2009 Mar]. Available from: www.ncbi.nlm.nih.gov.

19. NACE. Laboratory corrosion testing of metals in static chemical

20. cleaning solution at Tempera. Document number TM-0193 [Internet]. Belmont CA: Document Center Inc.; 2000.

21. Bakkalli F, Averbeck S, Averbeck D, et al. Biological effects of essential oils–A review. Food and Chem. Toxicology 2008; 46: 446–475.

22. Moraes VRS, Nogueira PCL, Gomes SVF. Aspectos químicos e biológicos do gênero Lippia enfatizando Lippia gracilis Schauer (Spanish) [Chemical and biological aspects of the genus Lippia emphasizing Lippia gracilis Schauer]. Eclética Química. 2011; 36: 64–77.

23. Dewick PM. Medicinal natural products: A biosynthetic approach. Hoboken, New Jersey: John Wiley & Sons, Inc., University of Nottingham, UK.; 2011.

24. Biswas K, Taylor MW, Turner SJ. Successional development of biofilms in moving bed biofilm reactor (MBBR) systems treating municipal wastewater. Appl. Microb. Biotec. 2013; 13: 5082–5088.

25. Leja K, Czaczyk K, Myszka K. The ability of Clostridium bifermentans strains to lactic acid biosynthesis in various environmental conditions. SpringerPlus 2013; 2: 44.

26. Palaniappan B, Toleti SR. Characterization of microfouling and corrosive bacterial community of a firewater distribution system. J. Biosc. Bioeng. 2016; 4: 435–441.

27. Albuquerque CC, Camara TR, Mariano RLR, et al. Antimicrobial action of the essential oil of Lippia gracilis Schauer. Brazilian Archiv. of Biol. and Techn. 2006; 49: 527–535.

28. Silva JPL, Duarte-Almeida JM, Perez DV, et al. Oregano essential oil: influence of the chemical composition on the inhibitory activity against Salmonella Enteritidis. Food Sci. Technol 2010; 30: 136–141.

29. Marques JM, Almeida FP, Lins U, et al. Nitrate treatment effects on bacterial community biofilm formed on carbon steel in produced water stirred tank bioreactor. World J. Microb. Biotechn. 2012; 28: 2355–2363.

30. Fadel F, Ben Hmamou1 D, Salghi1 R, et al. Antifungal activity and anti-corrosion inhibition of Origanum compactum extracts. Internat. J. Electrochem. Sci. 2013; 8: 11019–11032.

31. Ferreira RS, Napoleão TH, Santos AFS, et al. Coagulant and antibacterial activities of the water-soluble seed lectin from Moringa oleifera. Letters Appl. Microb. 2011; 53: 186–192.

32. Acevedo MS, Puentes C, Carreño K, et al. Antifouling paints based on marine natural products from Colombian Caribbean. J. Internat. Biodet. Biodeg. 2013; 83: 97–104.

33. Moura MC, Pontual EV, Paiva PMG, et al. Na outline to corrosive bacteria. In: Méndez-Vilas A (editor). Microbial pathogens and strategies for combating them: Science, technology and education. Paris: Formatex; 2013.

34. Zaferani SH, Sharifi M, Zaarei D, et al. Application of eco-friendly products as corrosion inhibitors for metals in acid pickling processes–A review. J. Environ. Chem. Engin. 2013; 1: 652–657.

35. Usher KM, Kaksonen AH, Cole I, et al. Critical review: Microbially influenced corrosion of buried carbon steel pipes. J. Internat. Biodet. Biodeg. 2014; 93: 84–106.

36. Gentil V. Corrosão (Português) [Corrosion]. 6th ed. Rio de Janeiro: LTC; 2011. p. 360.