Dear Colleagues,

Filtration is a method for separating chemicals by using the difference in the speed of their passage through the bed of a porous and semi-solid material. This method is especially useful for separating enzymes, proteins, peptides and amino acids from each other and from low molecular weight substances. Gel filtration is known as size exclusion chromatography or molecular sieve chromatography. The separation is based on the different molecular size present in the sample to enter the pores of the filtration gel medium. The stationary phase in this technique consists of beads of a hydrated, sponge-like material that has pores with molecular dimensions and a narrow range of sizes. By passing an aqueous solution, containing molecules of different sizes, through a column containing Molecular sieves are passed, smaller molecules enter the pores of the gel and move slowly in the column, and molecules that are larger than the pores of the filter medium move quickly in the column. Molecules are eluted in order of decreasing molecular size. Its main applications include:

Separation of Proteins and Peptides, Size-Exclusion Reaction Chromatography, Separation of Nucleic Acids and Nucleotides, Endotoxin Removal, Absolute Size-Exclusion Chromatography, Molecular Mass Estimation.

Research articles and reviews in this area of study are welcome.