Effects of enhanced UV-B radiation on the content of flavonoids in mesophyll cells of wheat

Shilong Ou, Shengqian Lu, Shenning Yang

Article ID: 369
Vol 1, Issue 1, 2018, Article identifier:

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The content of flavonoids in mesophyll cells of wheat was studied under the condition of enhanced UV-B radiation intensity. In this experiment, four groups of six days of control were treated with He-Ne laser group (L), enhanced UV-B radiation group (B), He-Ne laser and UV-B combined treatment group (B + L ), Normal light group (CK). Since the flavonoids carry some unsubstituted hydroxyl or glycosyl groups, it is a polar compound. By the 'similar compatibility' principle, they have some level of solubility in polar solvents, such as methanol, ethanol, n-butanol, propanol, and water. In this experiment, 70% ethanol was used to extract flavonoids. Finally, the total content of flavonoids in mesophyll cells was determined by visible spectrophotometry. The OD value of flavonoids was determined by rutin reagent 'The standard curves because rutin is a representative of flavonoids, it scavenging the role of free radicals significantly. The results showed that when the UV-B UV radiation intensity was enhanced, the content of flavonoids in wheat mesophyll cells increased, that is, the content of flavonoids in wheat leaves was higher than that in UV-B Strength was positively correlated. The results showed that the content of flavonoids in the mesophyll cells of the four control groups was the same as that of the B group> BL group> CK group> L group. With the prolonging of the treatment time of wheat, the content of flavonoids in wheat leaves at jointing-booting stage was significantly higher than that in seedling stage and panicle stage. This means that flavonoids are a protective substance that absorbs UV-B in plants, that is, the absorption of UV-B by flavonoids reduces the damage of UV-B to organs in plants [8] [10]; UV-B The smaller the damage, the less the content of flavonoids; laser damage caused by UV-B have a certain role in the repair. In this study, we further studied the effect of enhanced UV-B radiation on the content of flavonoids in mesophyll cells of wheat. The effects of UV-B radiation on the content of flavonoids in wheat were studied. Whether it has a very important significance for wheat has become a stress [5].

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Han Rong. He-Ne Laser Repairing Effect and Mechanism of Enhanced UV-B Radiation Damage in Wheat [Doctoral Dissertation]: Xi’an Northwestern University.

Hou Fujiang. Ben Guiying. Han fat. Analysis of the adaptation of plants to solar ultraviolet-B radiation. 1997.16 (2): P31, 35

Li Yuan. Lu Lu. Fang Zhixiang. Effect of SOD on Bacillus thuringiensis damaged by ultraviolet radiation. Henan Agricultural Sciences. 2003.2: P20, 23

Hou Fugujiang, Ben Guiying.Studies on the Effects of Ultraviolet-B Radiation on Plants. Acta Botanica Sinica. 1997.14 (4): P18, 23

Effects of UV-B Radiation on the Content and Distribution of Flavonoids in Wheat Varieties [J].

Chen Wanlong. Reflection and Absorption of Crops on Ultraviolet Radiation (UV) [J]. Chines Agricultural Meteorology, 1995, 16 (2): 9 ~ 12.

Lois R. Accumulation of UV - absorbing flavonoids induced by UV-B radiation in Arabidopsis thaliana L. I. Mechanisms of UV -resistance in Arabidopsis. Planta 1994, 194: 498 ~ 503.

Murali N S, Teramure A H. Effects of ultraviolet - b irradiance on soybean. VI. Influence of phosphorus nutrition on growth and flavonoid content. Physiol Plant, 1985, 63: 413 ~ 416.

Wang Chuanhai, He Duliang, Zheng Youfei, etc. At present, the impact of UV-B on wheat growth and yield in near-surface UV radiation, the Journal of Agricultural Environmental Science, 2003, 22 (2): 147-149.

Fang Debiao, Deng Zhenyong, Qiu Huimin, et al.Study on Soil Moisture Supplement in Dryland Farmland in Eastern Gansu [J]. Agricultural Research in Arid Land, 1996, 16 (2): 41 ~ 47.

ZOU Feng-shou-sheng YE Wan-zhi LU Gang Advances in Flavonoid Compounds in Plant Stress Reaction Cell Biology 2004.2 Vol.26 No.1

CHALKE SL.Environmental significance of anthocyanins in plant stress response [J]. Photochem Photobiol, 1999, 70: 1-9.

YIN Li-guo ZENG Fan-kun ZHONG Geng ZHENG Lian-jie Research status of bio-flavonoids in buckwheat food and oil 2002

Zhang Zhiliang Qu Weijing Plant Physiology Experimental Guidance Higher Education Press 2004/3 Third Edition

Harborne, J. B. (1967a). ‘Comparative Biochemistry of the Flavonoids’, Academic Press, London and New York.

Niklas, K.J. and Gianassi, D.E. (1978) .Am.J.Bot. 65.943.

Seshadri, T.R (1962). In The Chemistry of Flavonoid Compounds (ed.T.A.Geissman), p.6. Pergamon Press, New York.

Beck, A.B. (1964), Aust. J. Agric. Res. 15.223.

Trim, A.R. (1955). In Modern Methods of Plant Analysis (des. K. Paech and M.V. Seprace), Vol. 2, p. 295. Springer-Verlag,

Mukerjee, S.K., Sarkar, S.C. and Seshadri, T. R. (1969), Tetrahedron 25, 1063

DOI: http://dx.doi.org/10.24294/irr.v1i1.369


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