Cloning of Mouse β-actin Gene

Chengjun Ji, Mingli Wang, Xuedong Wen

Abstract


β-actin gene is a kind of actin, in a variety of cells and tissues, β-actin gene expression is relatively stable [1], and β-actin gene in nuclear DNA has multiple copies, increased The detection rate of nuclear DNA [2], which is often used as an internal reference in PCR. In this experiment, the β-actin gene with good purity was obtained by PT-PCR, amplification, cloning, screening and PCR identification and restriction enzyme digestion of the RNA extracted from the mouse liver, which laid the foundation for the later experiment.

 


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References


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http: //www.baidu.com Baidu Encyclopedia

http://jpkc.gdpu.cn/2jpfzswx/Course/Content.asp?c=345u0026a=587u0026todo=show plasmid DNA extraction and purification of alkaline lysis of Guangdong Institute of Molecular Biology Course website.


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