The Effects of Different Vitis vinifera Extracts Against Oxidative Stress Induced in Saccharomyces cerevisiae with Fenton Reagent

Ayse Dilek Ozsahin 1, Oguz Ayhan KIRECCI 2

Article ID: 635
Vol 1, Issue 1, 2018

VIEWS - 177 (Abstract) 16 (PDF)

Abstract


Grape (Vitis vinifera) is a rich source of phenolic compounds widely grown across the world. The aim of the present study is to evaluate the role Vitis vinifera in protection of cellular compounds against reactive oxygen species modification. In addition this research was to evaluate and compare influence of H2O2 and FeCl2H2O treatments on fatty acids, phytosterols, vitamins and glutathione contents of grape juice in Saccharomyces cerevisiae. The results indicate that, S. cerevisiae yeast synthesized biochemical compounds such as fatty acids, phytosterols, vitamins and glutathione in distinct quantities in a different culture environment. It was concluded that these differences in quantities of chemical compounds resulted from the fact that grape samples which were added to the growth-media of S. cerevisiae instead of glucose caused some differenced in these biochemical compounds synthesis. Different grape varieties contain varying ratios of substances as food content. Particularly a different carbon sources has an inhibiting or stimulating effect on enzyme activities which are responsible for fatty acids, phytosterols, vitamins and glutathione synthesis and cause a difference in these compounds quantities. 


Keywords


Saccharomyces cerevisiae; Grape; H2O2; Fatty Acid; Phytosterol; Vitamin

Full Text:

PDF


References


1. German JB, Frankel EN, Waterhouse AL, et al. Wine phenolics and targets of chronic diseases. In Watkins TR (ed.), Wine nutritional and therapeutic benefits Washington, DC: American Chemical Society. 1997; 196-214.

2. Katalinic V, Milos M, Kulisic T, et al. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry 2006; 94: 550-557.

3. Poudel PR, Tamura H, Kataoka I, et al. Phenolic compounds and antioxidant activities of skin and seeds of five wild grapes and two hybrids native to Japan. Journal of Food Composition and Analysis 2008; 21: 622-625.

4. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiological Reviews 1979; 59: 527-605.

5. Branco MR, Marinho HS, Cyrne L, et al. (2004). Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 in Saccharomyces cerevisiae. Journal of Biological Chemistry 2004; 279: 6501-6506.

6. Sousa-Lopes A, Antunes F, Cryne L, et al. Decreased cellular permeability to H2O2 protects Saccharomyces cerevisiae cells in stationary phase against oxidative stress. FEBS Letters 2004; 578: 152-156.

7. Folmer V, Pedroso N, Matias AC, et al. H2O2 induces rapid biophysical and permeability changes in the plasma membrane Saccharomyces cerevisiae. Biochimica et Biophysica Acta 2008; 1778: 1141–1147.

8. Antunes F, Cadenas E. Estimation of H2O2 gradients across biomembranes. FEBS Letters 2000; 475: 121-126.

9. Jamieson DJ. Oxidative stres responses of the yeast Saccharomyces cerevisia. Yeast 1998; 14: 1511-1527.

10. Izawa S, Inoue Y, Kimura A. Oxidative stres response in yeast: effect of glutathione on adaption to hydrogen peroxide stres in Saccharomyces cerevisia. FEBS Letters 1996; 368: 73-76.

11. Torija MJ, Beltran G, Novo M, et al. Effects of fermentation temperature and Saccharomyces species on the cell fatty acid composition and presence of volatile compounds in wine. International Journal of Food Microbiology 2003; 85: 127-136.

12. Dawson L, Boopathy R. Use of postharvest sugarcane residue for ethanol production. Bioresource Technology 2006; 98: 1695–1699.

13. Cipak A, Hasslacher M, Tehlivets O, et al. Saccharomyces cerevisiae strain experssing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stres induced by lipid peroxidation. Free Radical Biology and Medicine 2005; 40: 897–906.

14. Ozsahin AD, Guvenc M, Yilmaz O, et al. The effects of different sugar sources on fatty acid biosynthesis in the Saccharomyces cerevisiae cell culture. Journal of Animal and Veterinary Advances 2009; 8(3): 424–429.

15. Dilsiz N, Celik S, Yilmaz O, et al. The effects of selenium, vitamin E and their combination on the composition of fatty acids and proteins in Saccharomyces cerevisiae. Cell Biochemistry and Function 1997; 15: 265–269.

16. Hara A, Radın NS. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 1978; 90(1): 420–426.

17. Christie WW. Gas chromatography and lipids. Glaskow: The Oil Pres. 1992.

18. Katsanidis E, Addis PB. Novel HPLC analysis of tocopherols and cholesterol in tissue. Free Radical Biology and Medicine 1999; 27(11-12): 1137-1140.

19. Lowry OH, Rosenbrough NJ, Farr AL, et al. Protein measurement with the Folin-phenol reagent. The Journal of Biochemistry 1951; 193: 265-277.

20. Elman GI. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 1959; 70-77.

21. Gokturk Baydar N, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15: 335-339.

22. Bozan B, Tosun G, Özcan D. Study of polyphenol content in the seeds of red grape (Vitis vinifera L.) varieties cultivated in Turkey and their antiradical activity. Food Chemistry 2208; 109(2): 426-430.

23. Matias AC, Pedroso N, Teodoro N, et al. Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells in to H2O2. Free Radical Biology and Medicine 2007; 43: 1458-1465.

24. Mohamed AH, Chirala SS, Mody NH, et al. Primary structure of the multifunctional alpha subunit protein of yeast fatty acid synthase derived from FAS2 gene sequence. Journal of Biological Chemistry 1988; 263: 12315-12325.

25. McDonough VM, Roth TM. Growth temperature affects accumulation of exogenous Fatty acids and fatty acid composit in Schizosaccharomyces pombe. Antonie Van Leewenhoek 2004; 86: 349–354.

26. Ntambi JM Regulations of Stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lipid Research 1999; 409: 1549-1558.

27. Ammar B, Nouairi I, Zarrouk M, et al. The effect of cadmium on lipid and fatty acid biosynthesis in tomato leaves. Biologia 2008; 63(1): 86-93.

28. Tan T, Zhang M, Gao H. Ergosterol production by fed-batch fermentation of Saccharomyces cerevisiae. Enzyme and Microbial Technology 2003; 33: 366-370.

29. Zhang JF, Lıub H, Sun YY, et al. Responses of the antioxidant defenses of the Goldfish Carassius auratus, exposed to 2, 4 - dichlorophenol. Environmental Toxicology and Pharmacology 2005; 19: 185–190.

30. Qian SG. Ergosterol production in 5 l bottle shaken-flask experiments. China Journal Ind Microorganism 1998; 18(5): 18–23.

31. Kagan IA, Michel A, Prause A, et al. Gene transcription profiles of Saccharomyces cerevisiae after treatment with plant protection fungicide that inhibit ergosterol biosynthesis. Pesticide Biochemistry and Physiology 2005; 82: 133–153.

32. Zinser E, Paltauf F, Daum G. Sterol composition of yeast organelle membranes and subcellular distribution of enzymes involved in sterol metabolism. Journal of Bacteriology 1993; 175: 2853–2858.

33. Cobon GS, Haslam JM. The effect of altered membrane sterol composition on the temperature dependence of yeast mitochondrial ATPase. Biochemical and Biophysical Research Communications 1973; 52: 320–326.

34. Higgins VJ, Beckhouse AG, Oliver AD, et al. Yeast ge-nomewide expression analysis identifies a strong ergosterol and oxidative stres response during the initial stages of an industrial lager fer-mentation. Applied and Environmental Microbiology 2003; 69: 4777-4787.

35. Montañes FM, Pascual-Ahuir A, Proft M. Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors. Molecular Microbiology 2011; 79(84): 1008-1023.

36. Beutler E. Nutritional and metabolic aspects of glutathione. Annual Reviwe and Nutrition 1989; 9: 287-302.

37. Sies H. Glutathione and its role in cellular functions. Free Radical Biology Medcine 1999; 27: 916–921.

38. Penninckx M. A short review on the role of glutathione in the response of yeasts to nutritional, environmental, and oxidative stresses. Enzyme and Microbial Technology 2000; 26: 737-742.




DOI: https://doi.org/10.24294/ajb.v1i1.635

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Asian Journal of Botany

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License

This site is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.